FlyBase Allele Report: Dmel\sws[JF03428]

General Information

Symbol

Dmel\sws^JF03428

Species

D. melanogaster

Name

FlyBase ID

FBal0244963

Feature type

allele

Associated gene

Dmel\sws

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03428}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03428}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03428

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of hereditary spastic paraplegia

CEA

(Dutta et al., 2016)

model of blindness

CEA

(Kmoch et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cell body rind of adult lamina | adult stage | progressive, with Scer\GAL4^loco.PU

(Dutta et al., 2016)

eye photoreceptor cell | progressive, with Scer\GAL4^GMR.PU

(Kmoch et al., 2015)

giant fiber neuron | progressive, with Scer\GAL4^repo.PU

(Dutta et al., 2016)

Detailed Description

Statement

Reference

Adults expressing sws^JF03428 under the control of Scer\GAL4^loco.PU show a progressive decrease in locomotion in fast phototaxis assays.

(Sunderhaus et al., 2019)

14 day old Scer\GAL4^loco.PU>sws^GD3277 flies have vacuoles in the lamina cortex.

Expression of sws^JF03428 driven by Scer\GAL4^repo.PU or Scer\GAL4^loco.PU significantly reduces performance in a phototaxis assay (measuring percentage transitions towards light) in 2 day old flies compared to controls; this phenotype is progressive, with performance in 14 day old flies worse than in 2 day old flies.

Scer\GAL4^repo.PU>sws^JF03428 flies show a significant decline in activity and walking speed but not visual orientation (Buridan's paradigm) at 7 or 14 (but not 3) days old, compared to age-matched controls. Electroretinogram (ERG) recordings from young or old Scer\GAL4^repo.PU>sws^JF03428 flies did not detect any significant differences from controls.

Short latency response times between electrical stimulation of the giant fiber neuron and flight muscle (dorsal longitudinal muscle) responses are similar to age-matched controls in 3 or 14 day old Scer\GAL4^repo.PU>sws^JF03428 flies; Scer\GAL4^repo.PU>sws^JF03428 flies do show a significant increase in response latency in 14 day old flies (compared to 3 day olds), unlike controls (no significant difference). Reliability in response to high-frequency stimulation is also significantly affected in 14 day old Scer\GAL4^repo.PU>sws^JF03428 flies.

(Dutta et al., 2016)

Photoreceptors of Scer\GAL4^GMR.PU sws^JF03428 flies develop normally and appear unaffected in 7-day-old flies, but 30 days old flies show obvious signs of degeneration and some photoreceptors are lost. Raising these flies in the dark does not prevent this degeneration. Electroretinograms are normal in both young and aged mutant flies.

(Kmoch et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^loco.PU, sws^JF03428 has abnormal locomotor behavior | adult stage phenotype, suppressible by Hsap\PNPLA6^UAS.cTa, Scer\GAL4^loco.PU

(Sunderhaus et al., 2019)

Scer\GAL4^loco.PU, sws^JF03428 has abnormal locomotor behavior | adult stage phenotype, suppressible by Hsap\PNPLA6^T629R.UAS, Scer\GAL4^loco.PU