FlyBase Allele Report: Dmel\kirre[RNAi.UAS]

General Information

Symbol

Dmel\kirre^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0246204

Feature type

allele

Associated gene

Dmel\kirre

Associated Insertion(s)

Carried in Construct

P{UAS-kirre.RNAi}

Also Known As

UAS-kirre-IR

Key Links

Transgenic product class

RNAi_reagent

(Bao et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Bao et al., 2010)

Mutagen

in vitro construct

(Bao et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-kirre.RNAi}

Cytology

Description

A 500bp cDNA fragment of kirre (2505-2004) is cloned in an inverted repeat downstream of UAS regulatory sequences.

(Bao et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

kirre

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Bao et al., 2010)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Bao et al., 2010)

interommatidial cell, with Scer\GAL4^GMR.PF

(Bao et al., 2010)

interommatidial precursor cell, with Scer\GAL4^GMR.PF

(Bao et al., 2010)

ommatidium, with Scer\GAL4^GMR.PF

(Bao et al., 2010)

Detailed Description

Statement

Reference

Expression of kirre^{dsRNA.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF causes mild eye defects. Typically, bristle groups and inter-ommatidial cells are mis-positioned although ommatidia remain well separated as in wild-type.

(Bao et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of abnormal cell adhesion | somatic clone phenotype of Scer\GAL4^GMR.PF, sns^RNAi.UAS

(Bao et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has eye phenotype, enhanceable by sns^RNAi.UAS/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has ommatidium phenotype, enhanceable by sns^RNAi.UAS/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has eye phenotype, enhanceable by hbs^RNAi.UAS.cBa/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has ommatidium phenotype, enhanceable by hbs^RNAi.UAS.cBa/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has interommatidial cell phenotype, enhanceable by hbs^RNAi.UAS.cBa/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has interommatidial precursor cell phenotype, enhanceable by hbs^RNAi.UAS.cBa/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has interommatidial bristle phenotype, enhanceable by hbs^RNAi.UAS.cBa/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has interommatidial cell phenotype, enhanceable by sns^RNAi.UAS/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has interommatidial precursor cell phenotype, enhanceable by sns^RNAi.UAS/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Scer\GAL4^GMR.PF, kirre^RNAi.UAS has interommatidial bristle phenotype, enhanceable by sns^RNAi.UAS/Scer\GAL4^GMR.PF

(Bao et al., 2010)

Enhancer of

Statement

Reference

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, rst^GD2582

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of interommatidial cell phenotype of Scer\GAL4^GMR.PF, rst^GD2582

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of interommatidial precursor cell phenotype of Scer\GAL4^GMR.PF, rst^GD2582

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, rst^GD2582

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, sns^RNAi.UAS

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of interommatidial cell phenotype of Scer\GAL4^GMR.PF, sns^RNAi.UAS

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of interommatidial precursor cell phenotype of Scer\GAL4^GMR.PF, sns^RNAi.UAS

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, sns^RNAi.UAS

(Bao et al., 2010)

NOT Enhancer of

Statement

Reference

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, hbs^RNAi.UAS.cBa

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is a non-enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, hbs^RNAi.UAS.cBa

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is a non-enhancer of interommatidial cell phenotype of Scer\GAL4^GMR.PF, hbs^RNAi.UAS.cBa

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is a non-enhancer of interommatidial precursor cell phenotype of Scer\GAL4^GMR.PF, hbs^RNAi.UAS.cBa

(Bao et al., 2010)

kirre^RNAi.UAS/Scer\GAL4^GMR.PF is a non-enhancer of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, hbs^RNAi.UAS.cBa

(Bao et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Reduction of both sns and kirre expression through kirre^{dsRNA.Scer\UAS} and sns^{dsRNA.Scer\UAS} expression under the control of Scer\GAL4^GMR.PF enhances slightly the patterning defects seen in kirre down-regulation. Deleted, extra cells ('cone contact cells') are frequently found in direct contact with cone cell quartets. Depletion occasionally leads to inter-ommatidial cells occurring in multiple rows between ommatidia. In each case, ommatidial spacing is nonetheless nearly indistinguishable from single knock-down of sns alone.

Reduction of both hbs and kirre expression through kirre^{dsRNA.Scer\UAS} and hbs^IR.Scer\UAS expression under the control of Scer\GAL4^GMR.PF enhances slightly the patterning defects seen in kirre down-regulation. Depletion occasionally leads to inter-ommatidial cells occurring in multiple rows between ommatidia. In each case, ommatidial spacing is nonetheless nearly indistinguishable from single knock-down of hbs alone.

Reduction of both rst and kirre expression through kirre^{dsRNA.Scer\UAS} and rst^GD2582 expression under the control of Scer\GAL4^GMR.PF significantly enhances the patterning defects seen in rst down-regulation: 36.6% of ommatidia are found in direct contact with another ommatidium. Inter-ommatidial cells show little evidence of sorting into a single file.

(Bao et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Kirre^RNAi

(Na et al., 2015)

kirre^RNAi.UAS

kirre^{dsRNA.Scer\UAS}

kirre^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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