Flies expressing Hsap\TOR1A^ΔE.Scer\UAS under the control of Scer\GAL4^tub.PU exhibit significantly more susceptibility to H[[2]]O[[2]]- or paraquat- induced oxidative stress than controls. Susceptibility to tunicamycin-induced stress is also increased but there is no difference in sensitivity to the protein sulphide bond reducing compound dithiothreitol (DTT). Flies expressing Hsap\TOR1A^ΔE.Scer\UAS under the control of Scer\GAL4^tub.PU are sensitive to the autophagy inhibitors 3-MA, Wortmannin and LY294002 in comparison with controls.

Flies expressing Hsap\TOR1A^ΔE.Scer\UAS in muscles (under the control of Scer\GAL4^C57) or in neurons (Scer\GAL4^elav-C155) become severely incapacitated when exposed to high temperatures (38[o]C), whereas the majority of control flies remain active.

Larvae expressing Hsap\TOR1A^ΔE.Scer\UAS under the control of Scer\GAL4^elav-C155 exhibit aberrant bouton morphologies: the number of type I synaptic boutons is similar to controls, but the boutons are bigger and have more abnormal protrusions. The average length of the boutons does not differ from controls but the average surface area, volume and number of T-shaped active zones per bouton are all increased.

Abnormal free dense bodies are observed at the presynaptic terminals of larvae of larvae expressing Hsap\TOR1A^ΔE.Scer\UAS under the control of Scer\GAL4^elav-C155. In contrast to controls, the post-synaptic subsynaptic reticulum is interrupted by small clearings in ~20% of larvae. The average area of the synapse is significantly increase compared with the average area of control synapses. There are increased number of functional synapses (ones with T-shaped active zones) and silent synapses (synapses lacking T-shaped active zones).

Flies expressing Hsap\TOR1A^ΔE.Scer\UAS in all neurons under the control of Scer\GAL4^elav-C155 show striking behavioural differences compared to controls flies when subjected to a 38[o]C temperature shift for 10 minutes, and these abnormalities become more pronounced with age. 3-day old flies exhibit impaired locomotor control at 38[o]C, and by day 6, 90% of flies are severely incapacitated within 1 minute of exposure to 38[o]C. Flies exhibit twisting movements, and convulsive-like leg and wing movements.

There is no significant difference in the number of type I synaptic boutons in larvae expressing Hsap\TOR1A^ΔE.Scer\UAS in all neurons under the control of Scer\GAL4^elav-C155 or in muscles under the control of Scer\GAL4^C57. However, synaptic boutons are enlarged and many have abnormal irregular shapes compared to the smooth rounded appearance of synaptic boutons observed in control animals. Defects are also observed at regions of synaptic contact including an absence of synaptic vesicle pools near some synapses, abnormal membrane structures at the synapse, free dense bodies at the presynaptic terminal (rather than anchored to the membrane), and a signifiant increase in synaptic density area compared with control boutons.

Flies expressing Hsap\TOR1A^ΔE.Scer\UAS in dopaminergic neurons under the control of Scer\GAL4^Ddc.PL show behavioural differences compared to controls flies when subjected to a 38[o]C temperature shift for 10 minutes. Over 90% of 12-day old flies exhibit loss of locomotor control at 38[o]C.

Post-synaptic expression of Hsap\TOR1A^ΔE.Scer\UAS under the control of Scer\GAL4^C57 in muscles results in an age- and temperature-dependent loss of locomotor control, with over 90% of 9-day old flies remaining on the bottom of the vial when subjected to 38[o]C temperatures during a 10 minute period.

Hsap\TOR1A^ΔE.UAS, Scer\GAL4^elav-C155 has abnormal locomotor behavior | heat sensitive phenotype, suppressible by Smox^UAS.cZa, Scer\GAL4^elav-C155

Hsap\TOR1A^ΔE.UAS, Scer\GAL4^elav-C155 has abnormal locomotor behavior | heat sensitive phenotype, suppressible by Hsap\SMAD2^UAS.cMa, Scer\GAL4^elav-C155

Hsap\TOR1A^ΔE.UAS, Scer\GAL4^elav-C155 has abnormal locomotor behavior | heat sensitive phenotype, non-suppressible by Mad^UAS.cNa, Scer\GAL4^elav-C155

Hsap\TOR1A^ΔE.UAS, Scer\GAL4^elav-C155 has abnormal locomotor behavior | heat sensitive phenotype, non-suppressible by Smox¹/Smox[+]

Hsap\TOR1A^ΔE.UAS, Hsc70-3^RNAi.UAS.cUa, Scer\GAL4^elav-C155 has short lived phenotype

Hsap\TOR1A^ΔE.UAS, Scer\GAL4^elav-C155, Xbp1^RNAi.UAS.cUa has short lived phenotype

Hsap\TOR1A^ΔE.UAS, Scer\GAL4^elav-C155, Xbp1^k13803 has short lived phenotype

Atf6^GD14782, Hsap\TOR1A^ΔE.UAS, Scer\GAL4^elav-C155 has short lived phenotype

Hsap\TOR1A^ΔE.UAS, PEK^RNAi.UAS.cUa, Scer\GAL4^elav-C155 has short lived phenotype