Flies expressing nsl1^NIG.4699R under the control of Scer\GAL4^Mef2.247 show a 25% reduction in learning compared to controls in a courtship conditioning assay.

Expression of wah^NIG.4699R with Scer\GAL4^C57 results in muscles that are easily overstretched and tend to snap upon dissection of late larvae, although sarcomere structure is not overtly affected. Ultrastructurally, these muscles show an increase in surface invaginations and the presence of prominent densely packed composite bodies, termed 'sausage bodies', composed of electron dense elongated tubules of the several micrometres long and of a constant diameter of 37+/-0.5nm. Sausage bodies are predominantly localised in the sarcoplasm and rarely in sarcomeres.

Expression of wah^NIG.4699R in late larval muscles using Scer\GAL4^C57 also induces ubiquitin aggregates/puncta in the muscles, predominantly in the sarcoplasm and rarely in sarcomeres.