FlyBase Allele Report: Dmel\Usp8[KO]

General Information

Symbol

Dmel\Usp8^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0246478

Feature type

allele

Associated gene

Dmel\Usp8

Associated Insertion(s)

TI{TI}Usp8^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Mukai et al., 2010)

Mutagen

recombination

(Mukai et al., 2010)

FLPase

(Mukai et al., 2010)

I-SceI

(Mukai et al., 2010)

ends-out gene targeting

(Mukai et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}Usp8^KO

Cytology

Description

The entire Usp8 gene has been replaced by a w^+* marker.

(Mukai et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(von Stockum et al., 2019)

ameliorates Parkinson's disease 2

modeled by park²⁵

(Mauri et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | somatic clone

(Xia et al., 2012)

lethal - all die during embryonic stage

(Mukai et al., 2010)

some die during embryonic stage

(Mukai et al., 2010)

some die during pupal stage | somatic clone

(Xia et al., 2012)

visible, with Usp8^UAS.cMa

(Mukai et al., 2010)

Phenotype Manifest In

egg chamber | germline clone

(Mathieu et al., 2022)

female germline cyst | germline clone

(Mathieu et al., 2022)

nucleus | decreased number | germline clone | oogenesis

(Mathieu et al., 2022)

wing margin bristle, with Usp8^UAS.cMa

(Mukai et al., 2010)

Detailed Description

Statement

Reference

Ubpy^KO mutants carrying Ubpy^Scer\UAS.cMa in the absence of a Scer\GAL4 driver can survive to adulthood. These adults show loss of wing margin bristles.

(Mukai et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Usp8^KO is rescued by Usp8^UASp.Tag:V5/Scer\GAL4^VP16.bam

(Mathieu et al., 2022)

Partially rescued by

Usp8^KO is partially rescued by Usp8^UAS.cMa

(Mukai et al., 2010)

Not rescued by

Usp8^KO is not rescued by Usp8^{C572A.UASp.Tag:V5}/Scer\GAL4^VP16.bam

(Mathieu et al., 2022)

Comments

Ubpy^Scer\UAS.cMa in the absence of a Scer\GAL4 driver partially rescues Ubpy^KO mutants, with the rescued animals surviving to adulthood.

(Mukai et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ubpy^KO

Usp8^KO

(Mathieu et al., 2022)

dUBPY K.O.

(Mukai et al., 2010)

usp8KO

(Xia et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close