FlyBase Allele Report: Dmel\SiaT[L22]

General Information

Symbol

Dmel\SiaT^L22

Species

D. melanogaster

Name

FlyBase ID

FBal0246662

Feature type

allele

Associated gene

Dmel\SiaT

Associated Insertion(s)

TI{TI}SiaT^L22

Carried in Construct

Also Known As

DSiaT^L22

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Repnikova et al., 2010)

ends-out gene targeting

(Repnikova et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}SiaT^L22

Cytology

Description

Deletion that removes most of the ST6Gal coding sequence. The design of the targeting construct used to generate this allele was aimed at creating a mutant allele with Avic\GFP expression driven by the ST6Gal promoter (by inserting the Avic\GFP coding sequence), however the Avic\GFP coding sequence was damaged by a short insertion of approximately 30bp during the targeting event, and there is no Avic\GFP expression in the mutant.

(Repnikova et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior (with SiaT^S23)

(Islam et al., 2013)

abnormal neuroanatomy | larval stage

(Repnikova et al., 2010)

paralytic | adult stage (with SiaT^S23)

(Islam et al., 2013)

paralytic | adult stage | heat sensitive

(Frappaolo et al., 2017)

paralytic | adult stage | recessive

(Islam et al., 2013)

Phenotype Manifest In

embryonic/larval neuromuscular junction

(Repnikova et al., 2010)

NMJ bouton | larval stage

(Repnikova et al., 2010)

wing

(Frappaolo et al., 2017)

Detailed Description

Statement

Reference

SiaT^L22 adults have a held-out wing phenotype and present heat sensitive paralysis (38[o]C).

(Frappaolo et al., 2017)

Homozygous ST6Gal^L22 mutant flies are paralysed at elevated temperatures.

ST6Gal^L22/ST6Gal^S23 mutant flies are paralysed at elevated temperatures.

ST6Gal^L22/ST6Gal^S23 mutant flies show locomotor defects; they take an increased amount of time to right themselves when momentarily knocked down by a gentle agitation.

(Islam et al., 2013)

Mutant larvae have a decreased number of muscle synaptic branches and boutons at the neuromuscular junction of the dorsal abdominal muscle 1 (which is innervated by motor neuron MN1-Ib).

(Repnikova et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

SiaT^S23/SiaT^L22 has abnormal locomotor behavior phenotype, enhanceable by Csas^MB04236/Df(3L)XS2182

(Islam et al., 2013)

SiaT^L22 has paralytic | adult stage | recessive phenotype, enhanceable by Csas²¹

(Islam et al., 2013)

NOT Enhanced by

Statement

Reference

SiaT^L22 has paralytic | adult stage | recessive phenotype, non-enhanceable by Csas²¹/Csas[+]

(Islam et al., 2013)

Enhancer of

Statement

Reference

SiaT[+]/SiaT^L22 is an enhancer of paralytic | adult stage | heat sensitive phenotype of Cog7^z-4495/Cog7^z-5797

(Frappaolo et al., 2017)

SiaT^S23/SiaT^L22 is an enhancer of abnormal locomotor behavior phenotype of Csas^MB04236/Df(3L)XS2182

(Islam et al., 2013)

SiaT^L22 is an enhancer of paralytic | adult stage | recessive phenotype of Csas²¹

(Islam et al., 2013)

NOT Enhancer of

Statement

Reference

SiaT^L22 is a non-enhancer of abnormal neurophysiology | larval stage phenotype of Csas²¹

(Islam et al., 2013)

ST6Gal[+]/SiaT^L22 is a non-enhancer of paralytic | adult stage | recessive phenotype of Csas²¹

(Islam et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous ST6Gal^L22 enhances the paralysis defect seen in Csas²¹ mutant flies at elevated temperatures. The time of onset of paralysis is shorter than in either Csas²¹ or ST6Gal^L22 mutants alone. One copy of ST6Gal^L22 does not enhance the homozygous Csas²¹ phenotype, and one copy of Csas²¹ is insufficient to enhance the ST6Gal^L22 mutant phenotype.

ST6Gal^L22/ST6Gal^S23 enhances the locomotor defects seen in Csas^MB04236/Df(3L)XS2182 mutant flies. The time taken for flies to right themselves after being knocked over is extended compared to either mutant alone.

Homozygous Csas²¹ enhances the reduction in EJP amplitude seen in homozygous ST6Gal^L22 mutant flies, producing a EJP similar in magnitude to that seen in homozygous Csas²¹ mutant flies.

(Islam et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

ST6Gal^t8 rescues the locomotor defects of ST6Gal^S23 larvae and rescues the shortened longevity, bang sensitivity and temperature sensitive paralytic phenotype of ST6Gal^S23 adults.

The reduced number of boutons at the neuromuscular junction of dorsal abdominal muscle 1 which is seen in ST6Gal^S23 larvae is partially rescued by ST6Gal^t8.

(Repnikova et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DSiaT^L22

(Scott et al., 2023, Frappaolo et al., 2017, Islam et al., 2013)

L22

(Repnikova et al., 2010)

ST6Gal^L22

SiAT^L22

(Jetti et al., 2023)

SiaT^L22

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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