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General Information
Symbol
Dmel\SiaTL22
Species
D. melanogaster
Name
FlyBase ID
FBal0246662
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DSiaTL22
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion that removes most of the ST6Gal coding sequence. The design of the targeting construct used to generate this allele was aimed at creating a mutant allele with Avic\GFP expression driven by the ST6Gal promoter (by inserting the Avic\GFP coding sequence), however the Avic\GFP coding sequence was damaged by a short insertion of approximately 30bp during the targeting event, and there is no Avic\GFP expression in the mutant.

Insertion components
TI{TI}SiaTL22
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

SiaTL22 adults have a held-out wing phenotype and present heat sensitive paralysis (38[o]C).

Homozygous ST6GalL22 mutant flies are paralysed at elevated temperatures.

ST6GalL22/ST6GalS23 mutant flies are paralysed at elevated temperatures.

ST6GalL22/ST6GalS23 mutant flies show locomotor defects; they take an increased amount of time to right themselves when momentarily knocked down by a gentle agitation.

Mutant larvae have a decreased number of muscle synaptic branches and boutons at the neuromuscular junction of the dorsal abdominal muscle 1 (which is innervated by motor neuron MN1-Ib).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

SiaTL22 has paralytic | recessive | adult stage phenotype, non-enhanceable by Csas21/Csas[+]

Enhancer of
Statement
Reference

SiaT[+]/SiaTL22 is an enhancer of paralytic | adult stage | heat sensitive phenotype of Cog7z-4495/Cog7z-5797

SiaTL22 is an enhancer of paralytic | recessive | adult stage phenotype of Csas21

NOT Enhancer of
Statement
Reference

ST6Gal[+]/SiaTL22 is a non-enhancer of paralytic | recessive | adult stage phenotype of Csas21

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Homozygous ST6GalL22 enhances the paralysis defect seen in Csas21 mutant flies at elevated temperatures. The time of onset of paralysis is shorter than in either Csas21 or ST6GalL22 mutants alone. One copy of ST6GalL22 does not enhance the homozygous Csas21 phenotype, and one copy of Csas21 is insufficient to enhance the ST6GalL22 mutant phenotype.

ST6GalL22/ST6GalS23 enhances the locomotor defects seen in CsasMB04236/Df(3L)XS2182 mutant flies. The time taken for flies to right themselves after being knocked over is extended compared to either mutant alone.

Homozygous Csas21 enhances the reduction in EJP amplitude seen in homozygous ST6GalL22 mutant flies, producing a EJP similar in magnitude to that seen in homozygous Csas21 mutant flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

ST6Galt8 rescues the locomotor defects of ST6GalS23 larvae and rescues the shortened longevity, bang sensitivity and temperature sensitive paralytic phenotype of ST6GalS23 adults.

The reduced number of boutons at the neuromuscular junction of dorsal abdominal muscle 1 which is seen in ST6GalS23 larvae is partially rescued by ST6Galt8.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)