FlyBase Allele Report: Dmel\Csas[MB04236]

General Information

Symbol

Dmel\Csas^MB04236

Species

D. melanogaster

Name

FlyBase ID

FBal0198269

Feature type

allele

Associated gene

Dmel\Csas

Associated Insertion(s)

Mi{ET1}Csas^MB04236

Carried in Construct

Also Known As

Csas^Mi

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Islam et al., 2013)

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{ET1}Csas^MB04236

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Mi{ET1}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:19,988,634..19,988,634 [-]

(Islam et al., 2013, Bellen, 2007.10.30)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior (with Df(3L)XS2182)

(Islam et al., 2013)

abnormal neurophysiology | larval stage

(Islam et al., 2013)

paralytic | adult stage

(Islam et al., 2013)

paralytic | adult stage (with Csas²¹)

(Islam et al., 2013)

paralytic | adult stage (with Df(3L)XS2182)

(Islam et al., 2013)

viable

(Islam et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Csas^MB04236 mutant flies are homozygous viable, without any noticeable morphological defects. However, the flies appear to be weak and short-lived, with flies living 43% less time compared with wild type controls.

Csas^MB04236 mutant flies are paralysed at elevated temperatures, with the onset of paralysis being within 3-7 minutes at 38[o]C.

Csas^MB04236/Csas²¹ mutant flies are paralysed at elevated temperatures.

Csas^MB04236/Df(3L)XS2182 mutant flies are paralysed at elevated temperatures.

No significant difference is detected in the resting membrane potential of muscle cells between Csas^MB04236 mutant and wild type larvae. Evoked Excitatory Junctional Potential (EJP) amplitude is reduced in Csas^MB04236 mutant larvae compared to wild type. The miniEJP is unaffected.

Csas^MB04236/Df(3L)XS2182 mutant flies show locomotor defects; they take an increased amount of time to right themselves when momentarily knocked down by a gentle agitation.

(Islam et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Csas^MB04236/Df(3L)XS2182 has abnormal locomotor behavior phenotype, enhanceable by SiaT^S23/SiaT^L22

(Islam et al., 2013)

Csas^MB04236 has paralytic | adult stage phenotype, enhanceable by para[+]/para^ts1

(Islam et al., 2013)

Csas^MB04236 has paralytic | adult stage phenotype, enhanceable by para^lk5/para[+]

(Islam et al., 2013)

Suppressed by

Statement

Reference

Csas^MB04236, para^lk5/para[+] has paralytic | adult stage phenotype, suppressible | partially by Dp(1;4)r⁺l

(Islam et al., 2013)

Csas^MB04236 has paralytic | adult stage phenotype, suppressible by Dp(1;4)r⁺l

(Islam et al., 2013)

Csas^MB04236 has paralytic | adult stage phenotype, suppressible | partially by sei¹/sei[+]

(Islam et al., 2013)

Enhancer of

Statement

Reference

Csas^MB04236/Df(3L)XS2182 is an enhancer of abnormal locomotor behavior phenotype of SiaT^S23/SiaT^L22

(Islam et al., 2013)

Suppressor of

Statement

Reference

Csas[+]/Csas^MB04236 is a suppressor of paralytic | adult stage phenotype of sei¹

(Islam et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

ST6Gal^L22/ST6Gal^S23 enhances the locomotor defects seen in Csas^MB04236/Df(3L)XS2182 mutant flies. The time taken for flies to right themselves after being knocked over is extended compared to either mutant alone.

One copy of para^ts1 enhances the paralysis phenotype seen in homozygous Csas^MB04236 mutant flies at elevated temperatures. Onset of paralysis occurs earlier than in Csas^MB04236 mutants alone.

One copy of para^lk5 enhances the paralysis phenotype seen in homozygous Csas^MB04236 mutant flies at elevated temperatures. Onset of paralysis occurs earlier than in Csas^MB04236 mutants alone. This enhancement is suppressed in the presence of a para duplication (Dp(1;4)r⁺l).

The presence of one copy of Dp(1;4)r⁺l partially suppresses the paralytic phenotype seen in homozygous Csas^MB04236 mutant flies. The time taken for paralysis to occur at elevated temperatures is increased compared to Csas^MB04236 alone.

One copy of sei¹ partially suppresses the paralytic phenotype seen in Csas^MB04236 mutant flies in response to elevated temperatures.

One copy of Csas^MB04236 partially suppresses the paralytic phenotype seen in sei¹ mutant flies in response to elevated temperatures.

(Islam et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Csas^MB04236/Df(3L)XS2182 is rescued by Csas^UAS.Tag:FLAG

(Islam et al., 2013)

Comments

Expression of Csas^{Scer\UAS.T:Zzzz\FLAG} (without a GAL4 driver) rescues the paralysis, locomotor phenotypes and defects in EJP amplitude seen in Csas^MB04236/Df(3L)XS2182 mutant flies at elevated temperatures. Increasing Ca[2+] concentration is also able to rescue the neurophysiology phenotype.

(Islam et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24683

w¹¹¹⁸; Mi{ET1}Csas^MB04236/TM6C, cu¹ Sb¹

Notes on Origin

Discoverer

Comments

Excision of the Mi{ET1} element restores the longevity defects.

(Islam et al., 2013)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CSAS^Mi

(Scott et al., 2023, Islam et al., 2013)

Csas^MB04236

(Bellen, 2007.10.30)

Csas^Mi

(Islam et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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