FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Mef2EnR.UAS
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General Information
Symbol
Dmel\Mef2EnR.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0246694
Feature type
allele
Associated gene
Dmel\Mef2
Associated Insertion(s)
Carried in Construct
P{UAS-Mef2.EnR}
Also Known As
UAS-Mef2.EnR
Key Links
Transgenic product class
dominant_negative_variant
(Blanchard et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Blanchard et al., 2010)
Mutagen
in vitro construct
(Blanchard et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Mef2.EnR}
Cytology
Description

UAS regulatory sequences drive expression of the the first 128 amino acid residues of Mef2 (which includes the MADS box and Mef2 domain) fused to the en repressor domain (amino acids 2-298). This is expected to result in a dominant-negative Mef2 protein.

(Blanchard et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mef2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of dominant-negative Mef2Scer\UAS.T:Rep-en, under the control of Scer\GAL4cry.PZ results in arrhythmia.

      (Vrailas-Mortimer et al., 2014)

      39% of flies expressing Mef2Scer\UAS.T:en-Rep under the control of Scer\GAL4P0.5.Pdf are arrhythmic with respect to locomotor activity and the remainder have weaker rhythms than control flies.

      Expression of Mef2Scer\UAS.T:en-Rep under the control of Scer\GAL4tim.Scer\UAS causes long locomotor activity rhythms, with a period of 25.4 +/- 0.7 hours. Half of the flies die within the first 5 days of the assay.

      Adult LN[[v]] neurons are present and project normally when Mef2Scer\UAS.T:en-Rep is expressed under the control of Scer\GAL4tim.Scer\UAS.

      (Blanchard et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Overexpression of p38bScer\UAS.cAa background, rescues the arrhythmia phenotype in a Mef2Scer\UAS.T:Rep-en expressing flies, from 84% of flies to 53% displaying arrhythmia.

      Expression of p38bScer\UAS.cAa under the control of Scer\GAL4cry.PZ results in an increased level of tau in cry-expressing neurons, which is further enhanced by inhibition of Mef2 through co-expression of Mef2Scer\UAS.T:Rep-en.

      (Vrailas-Mortimer et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Mef2EnR.Scer\UAS
      Mef2EnR.UAS
      Mef2Scer\UAS.T:Rep-en
      Mef2Scer\UAS.T:en-Rep
      Name Synonyms
      Secondary FlyBase IDs
        References (7)