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General Information
Symbol
Dmel\Mef2EnR.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0246694
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of the the first 128 amino acid residues of Mef2 (which includes the MADS box and Mef2 domain) fused to the en repressor domain (amino acids 2-298). This is expected to result in a dominant-negative Mef2 protein.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of dominant-negative Mef2Scer\UAS.T:Rep-en, under the control of Scer\GAL4cry.PZ results in arrhythmia.

39% of flies expressing Mef2Scer\UAS.T:en-Rep under the control of Scer\GAL4P0.5.Pdf are arrhythmic with respect to locomotor activity and the remainder have weaker rhythms than control flies.

Expression of Mef2Scer\UAS.T:en-Rep under the control of Scer\GAL4tim.Scer\UAS causes long locomotor activity rhythms, with a period of 25.4 +/- 0.7 hours. Half of the flies die within the first 5 days of the assay.

Adult LN[[v]] neurons are present and project normally when Mef2Scer\UAS.T:en-Rep is expressed under the control of Scer\GAL4tim.Scer\UAS.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Overexpression of p38bScer\UAS.cAa background, rescues the arrhythmia phenotype in a Mef2Scer\UAS.T:Rep-en expressing flies, from 84% of flies to 53% displaying arrhythmia.

Expression of p38bScer\UAS.cAa under the control of Scer\GAL4cry.PZ results in an increased level of tau in cry-expressing neurons, which is further enhanced by inhibition of Mef2 through co-expression of Mef2Scer\UAS.T:Rep-en.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Mef2EnR.Scer\UAS
Mef2EnR.UAS
Mef2Scer\UAS.T:Rep-en
Mef2Scer\UAS.T:en-Rep
Name Synonyms
Secondary FlyBase IDs
    References (6)