UASt regulatory sequences drive expression of a mutated form of 14-3-3ζ (Leu15-Ala-Glu is mutated to Gln15-Gln-Arg and Arg88-Val-Glu is mutated to Asn88-Val-Gln) which is tagged with Tag:FLAG.
Scer\GAL4elav.PU/14-3-3ζMM.UAS.Tag:FLAG fails to rescue 14-3-3ζ07103/14-3-3ζP1188
Scer\GAL4elav.PU/14-3-3ζMM.UAS.Tag:FLAG fails to rescue 14-3-3ζP1188