A 4.5kb genomic fragment from the dl 5' flanking region drives expression of a mutated dl coding sequence (amino acid replacement R189E) that is tagged at the C-terminal end with the Tag:nt1 epitope. The Hsp70 polyadenylation signal is also present.
dlM18.Tag:nt1 fails to rescue dl1
The cuticle phenotype of embryos derived from dl1 females is not rescued by dlM18.T:boss-NT1.