FlyBase Allele Report: Dmel\slpr[AAA.UASp.Tag:HA]

General Information

Symbol

Dmel\slpr^{AAA.UASp.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0246985

Feature type

allele

Associated gene

Dmel\slpr

Associated Insertion(s)

Carried in Construct

P{UASp-slpr.AAA}

Key Links

Transgenic product class

missense_variant

(Garlena et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Garlena et al., 2010)

Mutagen

in vitro construct

(Garlena et al., 2010)

Progenitor genotype

Carried in construct

P{UASp-slpr.AAA}

Cytology

Description

UASp regulatory sequences drive expression of a mutated form of slpr, which contains the amino acid replacements T287A, S291A and T295A, and which is tagged at the C-terminal end with two copies of Tag:HA.

(Garlena et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

slpr

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^pnr-MD237

(Gonda et al., 2012)

Phenotype Manifest In

adult thorax | dorsal, with Scer\GAL4^pnr-MD237

(Gonda et al., 2012)

dorsal ectoderm, with Scer\GAL4^pnr-MD237

(Gonda et al., 2012)

embryonic/first instar larval cuticle | dorsal, with Scer\GAL4^pnr-MD237

(Garlena et al., 2010)

scutellum, with Scer\GAL4^pnr-MD237

(Gonda et al., 2012)

Detailed Description

Statement

Reference

Some embryos expressing slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^pnr-MD237 show defects in dorsal closure with detachment of the dorsal ectoderm anteriorly.

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^pnr-MD237 results in adults with a cleft thorax phenotype and widened scutellum.

Flies expressing slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS and raised at 25[o]C show either normal survival (males) or only slightly altered survival (females) on exposure to 37[o]C heat shock compared to wild-type controls.

(Gonda et al., 2012)

Embryos expressing slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^pnr-MD237 show defects in dorsal closure.

(Garlena et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Stronach et al., 2014)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Stronach et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 does not increase susceptibility of Tak1²/+ females to E.coli challenge.

(Stronach et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS fails to rescue slpr⁹²¹

(Stronach et al., 2014)

slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS fails to rescue slpr^BS06

(Gonda et al., 2012)

slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS fails to rescue slpr^BS06

(Garlena et al., 2010)

slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS fails to rescue slpr^3P5

(Garlena et al., 2010)

slpr^{AAA.UASp.Tag:HA}/Scer\GAL4^arm.PS fails to rescue slpr⁹²¹

(Garlena et al., 2010)

Comments

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS further enhances the lethality associated with slpr^BS06 mutant males.

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS does not rescue the dorsal closure phenotype seen in slpr⁹²¹ mutant males. Some embryos exhibit head defects.

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} does not suppress the reduction in eye size seen when egr^GS9830 is expressed under the control of Scer\GAL4^GMR.PF.

(Stronach et al., 2014)

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS fails to rescue the lethality of slpr^BS06 animals.

(Gonda et al., 2012)

Expression of slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS fails to rescue the lethality of slpr^BS06, slpr⁹²¹ and slpr^3P5 mutants.

(Garlena et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58825

w^*; P{UASp-slpr.AAA}1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

slpr^{AAA.Scer\UAS.P\T.T:Ivir\HA1}

slpr^{AAA.UASp.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (4)

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