FB2021_01, released Feb 18, 2021

Allele: Dmel\slpr3P5

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General Information
Symbol
Dmel\slpr3P5
Species
D. melanogaster
Name
FlyBase ID
FBal0134001
Feature type
allele
Associated gene
Dmel\slpr
Associated Insertion(s)
Carried in Construct
Genomic Maps
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    X:8,175,085..8,175,085 [-]
    Nucleotide change:

    G8175085A

    Reported nucleotide change:

    G?A

    Amino acid change:

    W361term | slpr-PA; W361term | slpr-PB; W361term | slpr-PC; W361term | slpr-PD

    Reported amino acid change:

    W?term

    Comment:

    TGG to TGA nonsense mutation in Trp codon.

    Comment:

    TGG to TGA nonsense mutation in Trp codon

    (Polaski et al., 2006, Stronach, 2006.5.26, Stronach and Perrimon, 2002)
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
         
        Progenitor genotype
        Cytology
        Nature of the lesion
        Statement
        Reference

        Nucleotide substitution: G?A.

        (Polaski et al., 2006, Stronach, 2006.5.26)

        Amino acid replacement: W360term.

        (Polaski et al., 2006, Stronach, 2006.5.26)

        Mutation is within the kinase domain.

        (Stronach and Perrimon, 2002)

        Amino acid replacement: W?term.

        (Stronach and Perrimon, 2002)
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        slpr3P5 displays partial embryonic lethality; no male hemizygous escapers are observed.

        (Baril et al., 2009)

        slpr3P5/Y mutants (lacking zygotic slpr function) show embryonic lethality and may have a dorsal open phenotype. Mutant embryos derived from homozygous female germline clones (lacking both zygotic and maternal slpr function) show a more severe lethality and cuticle phenotype than zygotically mutant embryos.

        29% of slprBS06/slpr3P5 escaper adult females have terminalia defects, with truncation of structures posterior to tergite 7, and are unable to defecate or lay eggs. 36% of the escaper females have maxillary palp defects.

        (Polaski et al., 2006)

        slpr3P5/Y embryos derived from heterozygous females die during embryogenesis or larval stages. Dead embryos have a variable dorsal open phenotype. A paternal copy of slpr+ can rescue embryos derived from females containing slpr3P5 homozygous germ-line clones. Mutant embryos derived from females containing homozygous germ-line clones which do not receive a paternal copy of slpr+ develop large anterior holes, due to severe defects in dorsal closure.

        (Stronach and Perrimon, 2002)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Suppressed by
        Statement
        Reference

        slpr3P5 has lethal phenotype, suppressible by alphXS-88/alphXS-88

        (Baril et al., 2009)

        slpr3P5 has lethal phenotype, suppressible by alphS-331/alphS-331

        (Baril et al., 2009)
        Suppressor of
        Statement
        Reference

        slpr[+]/slpr3P5 is a suppressor of visible phenotype of Rac1GMR.PN

        (Stronach and Perrimon, 2002)
        Other
        Statement
        Reference

        pucE69/puc[+], slpr3P5 has partially lethal phenotype

        (Stronach and Perrimon, 2002)
        Phenotype Manifest In
        Suppressed by
        Statement
        Reference

        slpr3P5 has phenotype, suppressible by pucE69/puc[+]

        (Stronach and Perrimon, 2002)
        Suppressor of
        Statement
        Reference

        slpr[+]/slpr3P5 is a suppressor of eye phenotype of Rac1GMR.PN

        (Stronach and Perrimon, 2002)

        slpr[+]/slpr3P5 is a suppressor of ommatidium phenotype of Rac1GMR.PN

        (Stronach and Perrimon, 2002)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        pucE69/+ rescues slpr3P5/Y animals to adulthood. The mutant male flies are weakly viable with no gross morphological defects.

        (Stronach and Perrimon, 2002)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        slpr3P5 is rescued by slprUASp.cPa/Scer\GAL4arm.PS

        (Polaski et al., 2006)

        slpr3P5 is rescued by slpr+tSa

        (Stronach and Perrimon, 2002)
        Partially rescued by

        slpr3P5 is partially rescued by slprUASp.cPa/Scer\GAL4arm.PS

        (Garlena et al., 2010)

        slpr3P5 is partially rescued by Scer\GAL4arm.PS/slprUASp.Tag:HA

        (Garlena et al., 2010)
        Not rescued by

        slpr3P5 is not rescued by slprAAA.UASp.Tag:HA/Scer\GAL4arm.PS

        (Garlena et al., 2010)

        slpr3P5 is not rescued by slprTAA.UASp.Tag:HA/Scer\GAL4arm.PS

        (Garlena et al., 2010)

        slpr3P5 is not rescued by slprASA.UASp.Tag:HA/Scer\GAL4arm.PS

        (Garlena et al., 2010)

        slpr3P5 is not rescued by slprAAT.UASp.Tag:HA/Scer\GAL4arm.PS

        (Garlena et al., 2010)

        slpr3P5 is not rescued by Scer\GAL4arm.PS/slprSKLC.UASp.Tag:HA

        (Garlena et al., 2010)

        slpr3P5 is not rescued by slprSKLC.AAA.UASp.Tag:HA/Scer\GAL4arm.PS

        (Garlena et al., 2010)

        slpr3P5 is not rescued by slprSH3.UASp.Tag:HA/Scer\GAL4arm.PS

        (Garlena et al., 2010)

        slpr3P5 is not rescued by Scer\GAL4arm.PS/slprCterm.UASp.Tag:HA

        (Garlena et al., 2010)

        slpr3P5 is not rescued by slprΔSH3.UASp.Tag:HA/Scer\GAL4arm.PS

        (Garlena et al., 2010)

        slpr3P5 is not rescued by Scer\GAL4arm.PS/slprAVA.UASp.Tag:HA

        (Garlena et al., 2010)
        Comments

        The lethality of slpr3P5 mutants is partially rescued by expression of slprScer\UAS.P\T.cPa or slprScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS.

        The lethality of slpr3P5 mutants is not rescued by expression of slprAAA.Scer\UAS.P\T.T:Ivir\HA1, slprTAA.Scer\UAS.P\T.T:Ivir\HA1, slprASA.Scer\UAS.P\T.T:Ivir\HA1, slprSKLC.AAA.Scer\UAS.P\T.T:Ivir\HA1, slprSH3.Scer\UAS.P\T.T:Ivir\HA1, slprAVA.Scer\UAS.P\T.T:Ivir\HA1, slprΔSH3.Scer\UAS.P\T.T:Ivir\HA1, slprCterm.Scer\UAS.P\T.T:Ivir\HA1 or slprSKLC.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS.

        Expression of slprAAT.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS in slpr3P5 mutants results in the recovery of a few rescued adults and many more rescued animals are observed as pharate adults.

        (Garlena et al., 2010)
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (2)
        Reported As
        Symbol Synonym
        3P5
        (Stronach and Perrimon, 2002)
        slpr3P5
        (Garlena et al., 2010, Baril et al., 2009, Polaski et al., 2006)
        Name Synonyms
        Secondary FlyBase IDs
          References (5)