Embryos simultaneously co-expressing cal1^Scer\UAS.cSa, cid^Scer\UAS.cJa and Cenp-C^Scer\UAS.cHa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} already show mitotic defects during mitosis 15. Chromosome congression into a metaphase plate is always slower than normal and metaphase is usually prolonged. Chromosome segregation is abnormal in approximately half of anaphases, with the defect usually consisting of lagging centromeres. The distance between sister kinetochores in metaphase plates is scattered over a larger range in the mutant embryos compared to the range seen in controls.