FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\nejRNAi.UAS.cSa
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General Information
Symbol
Dmel\nejRNAi.UAS.cSa
Species
D. melanogaster
Name
FlyBase ID
FBal0247258
Feature type
allele
Associated gene
Dmel\nej
Associated Insertion(s)
Carried in Construct
P{UAS-nej.RNAi.S}
Key Links
Transgenic product class
RNAi_reagent
(Smolik and Jones, 2007)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Smolik and Jones, 2007)
Mutagen
in vitro construct
(Smolik and Jones, 2007)
Progenitor genotype
Carried in construct
P{UAS-nej.RNAi.S}
Cytology
Description

UAS regulatory sequences drive expression of a construct comprised of intron 7 of nej inserted between inverted segments of nej coding sequence.

(Smolik and Jones, 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
nej
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Huntington's disease
      modeled by Hsap\HTTN548.Q128.UAS
      (Martin et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of nejdsRNA.Scer\UAS in wandering third instar larvae driven by Scer\GAL4hs.PB results in chromosome condensation defects. Approximately 50% of the metaphase chromosomes from brains expressing nejdsRNA.Scer\UAS fail to condense properly and appear to shred.

      The number of mitotic cells in eye discs of third instar larvae expressing nejdsRNA.Scer\UAS driven by Scer\GAL4hs.PB is reduced only two- to threefold by hydroxyurea (HU), while the number of mitotic cells observed in the discs from control larvae is reduced between ten- and sixteenfold by HU treatment. Non-heat-shocked control discs all retain a robust checkpoint response to HU treatment.

      (Smolik and Jones, 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      nejRNAi.UAS.cSa is rescued by nejUAS.Tag:V5/Scer\GAL4hs.PB

      (Smolik and Jones, 2007)
      Not rescued by

      nejRNAi.UAS.cSa is not rescued by nejF2161A.UAS.Tag:V5/Scer\GAL4hs.PB

      (Smolik and Jones, 2007)
      Comments

      Expression of nejScer\UAS.T:SV5\V5 rescues the mitosis phenotypes in eye discs resulting from Scer\GAL4hs.PB-driven nejdsRNA.Scer\UAS expression.

      Expression of nejF2161A.Scer\UAS.T:SV5\V5 fails to rescue the mitosis phenotypes in eye discs resulting from Scer\GAL4hs.PB-driven nejdsRNA.Scer\UAS expression.

      (Smolik and Jones, 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      nejRNAi.UAS.cSa
      nejRNAi.UAS
      nejdsRNA.Scer\UAS
      nejdsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (6)