FB2026_02 , released June 18, 2026
Allele: Dmel\Syt1C2A-D3.4N.UAS
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General Information
Symbol
Dmel\Syt1C2A-D3.4N.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0247579
Feature type
allele
Associated gene
Dmel\Syt1
Associated Insertion(s)
Carried in Construct
P{UAS-Syt1.C2A-D3,4N}
Key Links
Transgenic product class
missense_variant
(Yoshihara et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Yoshihara et al., 2010)
Mutagen
in vitro construct
(Yoshihara et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Syt1.C2A-D3,4N}
Cytology
Description

UAS regulatory sequences drive expression of Syt1 which carries mutations in the Ca[2+] binding sites of the C2A domain (amino acid replacements D282N and D284N).

(Yoshihara et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Syt1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Syt1C2A-D3.4N.Scer\UAS under the control of Scer\GAL4elav.PLu in Syt1N13/Syt1AD4 embryos results in similar levels of evoked release and total charge transfer as wild-type controls when evoked release properties are recorded from muscle 6 following motor nerve stimulation in 4mM extracellular Ca[2+]. However, the asynchronous release seen in Syt1N13/Syt1AD4 embryos is even more prominent in Syt1N13/Syt1AD4 embryos expressing Syt1C2A-D3.4N.Scer\UAS under the control of Scer\GAL4elav.PLu. Syt1N13/Syt1AD4 embryos expressing Syt1C2A-D3.4N.Scer\UAS under the control of Scer\GAL4elav.PLu show an enhanced rate of spontaneous release compared to controls.

      At lower Ca[2+] concentrations, recordings from muscle 6 following motor nerve stimulation in embryos expressing Syt1C2A-D3.4N.Scer\UAS under the control of Scer\GAL4elav.PLu in a Syt1N13/Syt1AD4 background have larger synaptic currents than controls, not only in the asynchronous component, but also in synchronous release.

      Embryos expressing Syt1C2A-D3.4N.Scer\UAS under the control of Scer\GAL4elav.PLu in a Syt1N13/Syt1AD4 background show a similar level of hypertonic stimulated release as controls.

      (Yoshihara et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Syt1C2A-D3.4N.UAS/Scer\GAL4elav.PLu partially rescues Syt1AD4/Syt1N13

      (Yoshihara et al., 2010)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Syt 1D282N,D284N
      (Yoshihara et al., 2010)
      Syt1C2A-D3.4N.Scer\UAS
      Syt1C2A-D3.4N.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)