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General Information
Symbol
Hsap\GALEUAS.cSa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Sanders
FlyBase ID
FBal0247664
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hGALE
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt drives expression of Hsap\GALE.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Hsap\GALEScer\UAS.cSa under the control of Scer\GAL4Act5C.PI rescues the lethality caused by expression of GaleNIG.12030R under the control of Scer\GAL4Act5C.PI. The rescued males and females show normal fecundity (number of offspring produced when mated to wild-type flies of the opposite sex).

The short lived phenotype seen in adult males and females expressing GaleNIG.12030R under the control of Scer\GAL4Act5C.PI from the late larval or early-to-mid-pupal stage onwards (the temperature sensitive Scer\GAL80ts.αTub84B allele is used to regulate the timing of expression) in the presence of 175mM galactose is completely suppressed by expression of Hsap\GALEScer\UAS.cSa under the control of Scer\GAL4Act5C.PI.

Expression of Hsap\GALEScer\UAS.cSa using either Scer\GAL4arm.PS or Scer\GAL4Act5C.PI rescues the lethality of GaleΔy/Galef00624 animals. Rescued animals are viable, fertile, and appear morphologically normal.

Expression of Hsap\GALEScer\UAS.cSa using Scer\GAL4Act5C.PI rescues the lethality of GaleNIG.12030R, Scer\GAL4Act5C.PI animals. Rescued animals are viable, fertile, and appear morphologically normal.

Expression of Hsap\GALEScer\UAS.cSa using Scer\GAL4drm.7.1 partially rescues the lethality of GaleΔy/Galef00624 animals, whereas use of Scer\GAL4elav-C155 or Scer\GAL4c754 is completely insufficient to rescue or delay lethality of this genotype.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\GALEScer\UAS.cSa
Hsap\GALEUAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Sanders
Secondary FlyBase IDs
    References (2)