FlyBase Allele Report: Dmel\Raf[Hsp83.PD]

General Information

Symbol

Dmel\Raf^Hsp83.PD

Species

D. melanogaster

Name

Heat shock protein 83 promoter construct of Ding

FlyBase ID

FBal0247944

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

P{Hsp83-Raf.FL}

Key Links

Transgenic product class

wild_type

(Ding et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ding et al., 2010)

Mutagen

in vitro construct

(Ding et al., 2010)

Progenitor genotype

Carried in construct

P{Hsp83-Raf.FL}

Cytology

Description

An Hsp83 promoter drives expression of full-length phl.

(Ding et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp83

Encoded product / tool

Raf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

phl[+]/Raf^Hsp83.PD is an enhancer of ventral denticle belt | maternal effect | heat sensitive phenotype of tor^11D

(Ding et al., 2010)

Suppressor of

Statement

Reference

Raf^Hsp83.PD is a suppressor of embryonic abdominal segment 8 | maternal effect phenotype of trk¹

(Ding et al., 2010)

NOT Suppressor of

Statement

Reference

Raf^Hsp83.PD is a non-suppressor of abdominal 8 ventral denticle belt | maternal effect phenotype of tor^rv66

(Ding et al., 2010)

Raf^Hsp83.PD is a non-suppressor of embryonic abdominal segment 8 | maternal effect phenotype of trk¹

(Ding et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The denticle belt phenotype seen in larvae derived from derived from tor^11D/+ females at 29[o]C is enhanced by phl^Hsp83.PD (penetrance is increased to 31.8%) and, to a lesser degree, by phl^ΔN114.Hsp83 (penetrance is increased to 17.1%).

phl^Hsp83.PD partially restores abdominal segment 8 denticle belts in embryos derived from homozygous trk¹ females.

The lack of the abdominal segment 8 denticle belts seen in embryos derived from homozygous tor^rv66 females is not rescued by phl^Hsp83.PD.

(Ding et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Raf^Hsp83.PD rescues Raf¹¹

(Ding et al., 2010)

Comments

phl^ΔN114.Hsp83 can rescue the posterior defects seen in embryos derived from derived from females containing homozygous phl¹¹ germline clones (rescue is seen if the level of expression from the transgene is equal or greater than the endogenous wild-type level of phl protein), but phl^ΔN114.Hsp83 is less active than phl^Hsp83.PD in this assay (the latter can rescue the posterior defects at much lower levels of expression).

(Ding et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Raf^Hsp83.PD

phl^Hsp83.PD

Name Synonyms

Heat shock protein 83 promoter construct of Ding

Secondary FlyBase IDs

References (1)

Report Sections

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