FlyBase Allele Report: Dmel\cnn[PA.UASp.GFP]

General Information

Symbol

Dmel\cnn^PA.UASp.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0247976

Feature type

allele

Associated gene

Dmel\cnn

Associated Insertion(s)

Carried in Construct

P{UASp-GFP-cnn.PA}

Key Links

Transgenic product class

wild_type

(Eisman et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Eisman et al., 2009)

Mutagen

in vitro construct

(Eisman et al., 2009)

Progenitor genotype

Carried in construct

P{UASp-GFP-cnn.PA}

Cytology

Description

UASp regulatory sequences drive expression of the cnn-PA isoform, which is tagged with GFP.

(Eisman et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

cnn

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, cnn^mfs3

(Eisman et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

The number of adults that develop to adulthood represents only 32% of eggs laid by cnn^mfs3 mutant females that also express cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Eisman et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{VP16.nanos.UTR}/cnn^PA.UASp.GFP rescues cnn^mfs7

(Eisman et al., 2009)

Scer\GAL4^{VP16.nanos.UTR}/cnn^PA.UASp.GFP rescues cnn^mfs3

(Eisman et al., 2009)

Fails to rescue

Scer\GAL4^{VP16.nanos.UTR}/cnn^PA.UASp.GFP fails to rescue cnn^HK21

(Eisman et al., 2009)

Comments

Expression of cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues embryogenesis in cnn^mfs3 and in cnn^mfs7 animals; approximately 92% of eggs produced by cnn^mfs3 or cnn^mfs7 females expressing cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} hatch.

Expression of cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the cnn^HK21 mutant phenotype; none of the eggs produced by cnn^HK21 females expressing cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} hatch.

(Eisman et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

cnn^{PA.Scer\UAS.P\T.T:Avic\EGFP}

(Eisman et al., 2015)

cnn^{PA.Scer\UAS.P\T.T:Avic\GFP}

cnn^PA.UASp.GFP

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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