A roX1 cDNA with the entire RB1 to RB3 domain region deleted is cloned downstream of Hsp83 promoter sequences.
lncRNA:roX1-3RB.Hsp83 fails to rescue Df(1)52/lncRNA:roX1MB710
The presence of roX1ΔRB1-3.Hsp83 fails to rescue roX1MB710 Df(1)52 male viability to wild-type levels.