FlyBase Allele Report: Dmel\Osbp[1]

General Information

Symbol

Dmel\Osbp¹

Species

D. melanogaster

Name

FlyBase ID

FBal0248442

Feature type

allele

Associated gene

Dmel\Osbp

Associated Insertion(s)

TI{TI}Osbp¹

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Ma et al., 2010)

Mutagen

ends-out gene targeting

(Hartl and Scott, 2016.3.2, Ma et al., 2010)

site specific recombination

(Ma et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}Osbp¹

Cytology

Description

The Osbp gene has been replaced with a mini-w marker (w^+*).

(Hartl and Scott, 2016.3.2)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male semi-sterile

(Ma et al., 2010)

male sterile (with Df(3R)ED6220)

(Ma et al., 2010)

viable

(Ma et al., 2010)

Phenotype Manifest In

individualization stage spermatid

(Ma et al., 2010)

Detailed Description

Statement

Reference

Osbp¹ mutant seminal vesicles are devoid of mature sperm, and sperm debris is present in the basal region of the testis. Individualised elongated spermatids do not display a smooth appearance as in wild-type but show numerous blebs of unremoved cytoplasm along their length. The individualisation complex is mildly disorganised, displaying irregular shapes with few triangular cone structures. Many spermatids are enclosed within one membrane, rather than each spermatid in a single membrane as in wild-type.

Osbp¹ mutant males raised on regular medium are sterile. Osbp¹ mutants raised on medium supplemented with cholesterol recover some fertility. The mildly disorganised individualisation complexes observed in these animals is also rescued by additional sterols.

(Ma et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Osbp¹, fan^KO has male semi-sterile phenotype

(Ma et al., 2010)

Phenotype Manifest In