FlyBase Allele Report: Dmel\p38b[156A]

General Information

Symbol

Dmel\p38b^156A

Species

D. melanogaster

Name

FlyBase ID

FBal0248527

Feature type

allele

Associated gene

Dmel\p38b

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2010)

Mutagen

P-element activity

(Chen et al., 2010)

Progenitor genotype

P{SUPor-P}p38b^KG01337

(Chen et al., 2010)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 900bp deletion which removes the promoter region and 5' portion of p38b.

(Chen et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Chen et al., 2010)

abnormal immune response

(Chakrabarti et al., 2014, Chen et al., 2010)

increased mortality | conditional

(Li et al., 2020)

melanotic mass phenotype | drug conditional

(Chen et al., 2010)

Phenotype Manifest In

adult Malpighian tubule

(Li et al., 2020)

embryonic/larval hindgut | drug conditional

(Chen et al., 2010)

Detailed Description

Statement

Reference

p38b^156A adults exhibit lower secretion rate of Malpighian tubules in post-injury condition.

p38b^156A adults die faster upon clean injury.

(Li et al., 2020)

p38b^156A mutant flies are more susceptible to Ecc15 infection than wild-type flies.

No lipid accumulation is seen in the intestines of p38b^156A mutant flies.

(Chakrabarti et al., 2014)

p38b^156A homozygous mutants do not exhibit any heart defects.

(Lim et al., 2014)

Mutant flies appear morphologically normal, but development from egg deposition to eclosion takes two days longer than normal at 22[o]C.

Introduction of dextran sodium sulfate into food induces melanisation of the hindgut in mutant larvae (in contrast to wild-type larvae).

Mutant adults show some decrease in the rate of survival compared to wild-type controls after infection with either Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana.

(Chen et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

p38b^156A has increased mortality | conditional phenotype, suppressible by Scer\GAL4^Uro.PU/mat^UAS.EGFP

(Li et al., 2020)

Suppressor of

Statement

Reference

p38a¹³/p38b^156A is a suppressor of abnormal oxidative stress response phenotype of Cat^UAS.cAa, Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

(Lim et al., 2014)

Other

Statement

Reference

p38a¹³, p38b^156A has abnormal oxidative stress response phenotype

(Lim et al., 2014)

p38a¹³, p38b^156A has abnormal immune response phenotype

(Chen et al., 2010, Chen et al., 2010)

p38a¹³, p38b^156A has melanotic mass phenotype | third instar larval stage phenotype

(Chen et al., 2010)

p38a¹³, p38b^156A has melanotic mass phenotype | adult stage phenotype

(Chen et al., 2010)

p38a¹³, p38b^156A has melanotic mass phenotype phenotype

(Chen et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

p38a¹³/p38b^156A is a suppressor of adult heart phenotype of Cat^UAS.cAa, Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

(Lim et al., 2014)

Other

Statement

Reference

p38a¹³, p38b^156A has adult heart phenotype

(Lim et al., 2014)

p38a¹³, p38b^156A has adult hindgut phenotype

(Chen et al., 2010)

p38a¹³, p38b^156A has embryonic/larval hindgut | third instar larval stage phenotype

(Chen et al., 2010)

p38a¹³, p38b^156A has embryonic/larval hindgut phenotype

(Chen et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

p38a¹³, p38b^156A double mutant flies exhibit abnormal heart function, as characterised by an increased incidence of irregular beats, a broader distribution of HPs, and significantly elevated the arrhythmia index, compared to wild-type flies. The diastolic and systolic diameters are significantly smaller in p38a¹³, p38b^156A double homozygotes than in wild-type, resulting in a narrower heart tube.

A p38b^156A, p38a¹³ double mutant background partially restores a normal heart rhythm and normalizes the diastolic and systolic heart diameters of flies expressing Cat^Scer\UAS.cAa under the control of Scer\GAL4^Dot.PK.

(Lim et al., 2014)

Double mutant Mpk2¹³ p38b^156A late third instar larvae and adults show melanisation in the hindgut. Vacuoles are detected in the epithelial cells in the melanised regions.

Mpk2¹³ p38b^156A double mutant flies show a reduced survival rate compared to control flies after infection with either P. aeruginosa, S. aureus or A. fumigatus. The mutant flies show normal resistance to infection with E. coli.

(Chen et al., 2010)

Mpk2¹³ p38b^156A double mutant animals typically die at larval and pupal stages under standard culture conditions, with only a few escapers. Raising the double mutant animals under either near-sterile conditions or with standard food containing antibiotics results in a significant increase in survival rate to the pupal stage.

Mpk2¹³ p38b^156A double mutant animals show reduced survival to the pupal stage compared to controls when raised on sterilised food into which P. aeruginosa has been introduced. Introducing E. coli or S. cerevisiae into sterilised food has no effect on survival of Mpk2¹³ p38b^156A double mutants.

Double mutant Mpk2¹³ p38b^156A larvae show melanisation in the hindgut. There is a significant reduction in this melanisation if the animals are cultured in near-sterile conditions ot on standard food containing antibiotics. Introduction of P. aeruginosa into sterilised food increases the occurrence of melanisation in the Mpk2¹³ p38b^156A animals, while introduction of either E. coli or S. cerevisiae has no effect on melanisation in the hindgut.

Mpk2¹³ p38b^156A adults show a strongly reduced rate of survival compared to wild-type controls after infection with either Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana.

(Chen et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of p38b^Scer\UAS.cAa under the control of Scer\GAL4^da.G32 rescues the survival rate of Mpk2¹³ p38b^156A adults infected with either Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana to a level close to wild type.

(Chen et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

D-p38b^156A

(Lim et al., 2019)

p38b^156A

(Li et al., 2020, Kenmoku et al., 2017, Chakrabarti et al., 2014, Lim et al., 2014, Chen et al., 2010, Chen et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (7)

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