FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\lokUASp.Tag:HA
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General Information
Symbol
Dmel\lokUASp.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0248730
Feature type
allele
Associated gene
Dmel\lok
Associated Insertion(s)
Carried in Construct
P{UASp-lok.HA}
Key Links
Transgenic product class
wild_type
(Bakhrat et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bakhrat et al., 2010)
Mutagen
in vitro construct
(Bakhrat et al., 2010)
Progenitor genotype
Carried in construct
P{UASp-lok.HA}
Cytology
Description

UASp regulatory sequences drive expression of the entire lok ORF tagged with Tag:HA coding sequences.

(Bakhrat et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
lok
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of lokScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF has no effect on eye morphology.

      Overexpression of lokScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, which drives expression during ovarian stem cell development and also during oogenesis, has no effect on stem cell development but leads to massive cell death of stage 8/9 egg chambers in mid-oogenesis, visualized by nurse cell DNA condensation.

      Overexpression of lokScer\UAS.P\T.T:Ivir\HA1 in germ cells under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16, which drives expression during mid to late oogenesis and is loaded into eggs, causes cell death of stage 8/9 egg chambers, as visualized by nurse cell condensation. Overexpression has no effect on survival of the germline. Ovaries from lokScer\UAS.P\T.T:Ivir\HA1 overexpressing germlines show degeneration in 52% of stage 8/9 egg chambers.

      Overexpression of lokScer\UAS.P\T.T:Ivir\HA1 in the follicle somatic cells using Scer\GAL4GR1 does not induce germline cell death or egg chamber survival.

      Overexpression of lokScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in mid-stage egg chambers leads to both chromatin condensation and DNA fragmentation.

      Expression of lokScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in mid-stage egg chambers does not induce autophagic cell death. There is a delay in lysosome increase in these overexpression mutants.

      (Bakhrat et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      lokUASp.Tag:HA/Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF, p53UASp.cBa

      (Bakhrat et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of lokScer\UAS.P\T.T:Ivir\HA1 with p53Scer\UAS.P\T.cBa under the control of Scer\GAL4GMR.PF greatly enhances the p53Scer\UAS.P\T.cBa phenotype with almost complete loss of the eye.

      Co-expression of lokScer\UAS.P\T.T:Ivir\HA1 with p53Scer\UAS.P\T.cBa during mid-oogenesis under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 does not block lokScer\UAS.P\T.T:Ivir\HA1-dependent cell death. The level of cell death of egg chambers is similar as when lokScer\UAS.P\T.T:Ivir\HA1 is overexpressed alone.

      Expression of thScer\UAS.P\T.cPa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress mid-oogenesis lokScer\UAS.P\T.T:Ivir\HA1-mediated cell death.

      (Bakhrat et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Overexpression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not affect cell death caused by expression of lokScer\UAS.P\T.T:Ivir\HA1.

      Overexpression of NPV(Sl)\P49Scer\UAS.P\T.cBa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not affect cell death caused by expression of lokScer\UAS.P\T.T:Ivir\HA1.

      (Bakhrat et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      lokScer\UAS.P\T.T:Ivir\HA1
      lokUASp.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)