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General Information
Symbol
Dmel\Diap1UASp.cPa
Species
D. melanogaster
Name
FlyBase ID
FBal0150217
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UASp-DIAP1, UAS-diap1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences control expression of Diap1 (+421 to +1748 of Diap1 cDNA (GenBank: L49440)).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Engulfment of nurse cells by follicle cells is largely inhibited in the "undead" egg chambers (egg chambers in which the nurse cell nuclei fail to condense and the follicle cells disappear) of starved flies expressing thScer\UAS.P\T.cPa in the germline under the control of Scer\GAL4nos.PG. Most of the follicle cells fail to enlarge and display thinning out of the membranes. 36% of undead egg chambers show some FC enlargement.

Expression of thScer\UAS.P\T.cPa under the control of both Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 results in defective egg chambers during mid-oogenesis (stages 7-9), characterised by a lack of follicle cells and the presence of large, uncondensed nurse cell nuclei. Approximately 20% of stage 14 egg chambers show uncondensed, persisting nurse cell nuclei.

The base of the ovaries of starved females expressing thScer\UAS.P\T.cPa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is filled with a mass of unhealthy, undead egg chamber tissue, including large uncondensed nurse cell nuclei. Residual egg chambers are devoid of follicle cell nuclei.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Overexpression of thScer\UAS.P\T.cPa with p53Scer\UAS.P\T.cBa, during oogenesis under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the p53Scer\UAS.P\T.cBa-dependent stem cell loss.

thScer\UAS.P\T.cPa can partially suppress the sterility and egg chamber degeneration seen in adult females carrying one copy of P{UASp-Dcp-1.ΔN} and two copies of P{GAL4::VP16-nos.UTR} raised at 25oC. However, the resulting egg chambers sometimes lack follicle cells and/or have extra nurse cells.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Diap1Scer\UAS.P\T.cPa
Diap1UASp.cPa
thScer\UAS.P\T.cPa
Name Synonyms
Secondary FlyBase IDs
    References (7)