FlyBase Allele Report: Dmel\Gli[UAS.cPa]

General Information

Symbol

Dmel\Gli^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Padash-Barmchi

FlyBase ID

FBal0248971

Feature type

allele

Associated gene

Dmel\Gli

Associated Insertion(s)

Carried in Construct

P{UAS-Gli.P}

Key Links

Transgenic product class

wild_type

(Padash-Barmchi et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Padash-Barmchi et al., 2010)

Mutagen

in vitro construct

(Padash-Barmchi et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Gli.P}

Cytology

Description

UAS regulatory sequences drive expression of Gli.

(Padash-Barmchi et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Gli

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^ap-md544

(Padash-Barmchi et al., 2010)

increased cell number, with Scer\GAL4^ap-md544

(Padash-Barmchi et al., 2010)

increased occurrence of cell division | larval stage, with Scer\GAL4^ap-md544

(Padash-Barmchi et al., 2010)

Phenotype Manifest In

wing disc, with Scer\GAL4^ap-md544

(Padash-Barmchi et al., 2010)

Detailed Description

Statement

Reference

Expression of Gli^Scer\UAS.cPa in the wing imaginal disc under the control of Scer\GAL4^ap-md544 results in delamination of the columnar epithelial cells. Migration of these cells into the ventral compartment of the disc is observed. A significant increase in the number of dividing cells is observed in the dorsal compartment of the wing disc, and ectopic folds in the wing disc are seen. Cell death is observed in the wing disc, which is not evident in control discs.

(Padash-Barmchi et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gli^UAS.cPa, Scer\GAL4^ap-md544 has increased cell death phenotype, enhanceable by puc^JK0/puc[+]

(Padash-Barmchi et al., 2010)

Gli^UAS.cPa, Scer\GAL4^ap-md544 has increased cell number phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Padash-Barmchi et al., 2010)

Suppressed by

Statement

Reference

Gli^UAS.cPa, Scer\GAL4^ap-md544 has increased cell death phenotype, suppressible by bsk^DN.UAS, Scer\GAL4^ap-md544

(Padash-Barmchi et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gli^UAS.cPa, Scer\GAL4^ap-md544 has wing disc phenotype, enhanceable by puc^JK0/puc[+]

(Padash-Barmchi et al., 2010)

Suppressed by

Statement

Reference

Gli^UAS.cPa, Scer\GAL4^ap-md544 has wing disc phenotype, suppressible by bsk^DN.UAS, Scer\GAL4^ap-md544

(Padash-Barmchi et al., 2010)

Gli^UAS.cPa, Scer\GAL4^ap-md544 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Padash-Barmchi et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The migratory behaviour and apoptosis observed in wing disc cells expressing Gli^Scer\UAS.cPa under the control of Scer\GAL4^ap-md544 is enhanced in a puc^JK0/+ background.

Co-expression of bsk^DN.Scer\UAS in wing discs expressing Gli^Scer\UAS.cPa under the control of Scer\GAL4^ap-md544 completely suppresses cell migration and apoptosis.

(Padash-Barmchi et al., 2010)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa in wing discs expressing Gli^Scer\UAS.cPa under the control of Scer\GAL4^ap-md544 blocks cell migration, but overgrowth and ectopic folds in the dorsal wing disc are observed.

(Padash-Barmchi et al., 2010)

Complementation and Rescue Data

Rescues

Gli^UAS.cPa/Scer\GAL4^da.G32 rescues Gli^AE2Δ45

(Padash-Barmchi et al., 2010)

Comments

Expression of Gli^Scer\UAS.cPa under the control of Scer\GAL4^da.G32 fully rescues the embryonic lethality of Gli^AE2Δ45 mutants. Rescued embryos develop into larvae, but the majority of these become paralysed and die. Adult escapers exhibit malformed legs and fail to inflate their wings.

(Padash-Barmchi et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gli^Scer\UAS.cPa

Gli^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Padash-Barmchi

Secondary FlyBase IDs

References (1)

Report Sections

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