FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\GliFF.UAS
Open Close
General Information
Symbol
Dmel\GliFF.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0248973
Feature type
allele
Associated gene
Dmel\Gli
Associated Insertion(s)
Carried in Construct
P{UAS-Gli.FF}
Key Links
Transgenic product class
missense_variant
(Padash-Barmchi et al., 2010)
polypeptide_post_translational_processing_variant
(Padash-Barmchi et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Padash-Barmchi et al., 2010)
polypeptide_post_translational_processing_variant
(Padash-Barmchi et al., 2010)
Mutagen
in vitro construct
(Padash-Barmchi et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Gli.FF}
Cytology
Description

UAS regulatory sequences drive expression of Gli modified to carry two tyrosine substitutions (Y766F and Y790F), to mimic a constitutively-dephosphorylated state.

(Padash-Barmchi et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Gli
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of GliFF.Scer\UAS in the wing imaginal disc under the control of Scer\GAL4ap-md544 results in delamination of the columnar epithelial cells. Migration of these cells into the ventral compartment of the disc is observed. A significant increase in the number of dividing cells is observed in the dorsal compartment of the wing disc, and ectopic folds in the wing disc are seen. Cell death is observed in these wing discs, a phenotype that is not observed in control discs.

      (Padash-Barmchi et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of BacA\p35Scer\UAS.cHa in wing discs expressing GliFF.Scer\UAS under the control of Scer\GAL4ap-md544 blocks cell migration, and excessive overgrowth and ectopic folds in the dorsal wing disc are observed.

      (Padash-Barmchi et al., 2010)
      Complementation and Rescue Data
      Rescues

      GliFF.UAS/Scer\GAL4da.G32 rescues GliAE2Δ45

      (Padash-Barmchi et al., 2010)
      Comments

      Expression of GliFF.Scer\UAS under the control of Scer\GAL4da.G32 fully rescues the embryonic lethality of GliAE2Δ45 mutants. Rescued embryos develop into larvae, but the majority of these become paralysed and die. Adult escapers exhibit malformed legs and fail to inflate their wings.

      (Padash-Barmchi et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      GliFF.Scer\UAS
      GliFF.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)