FB2026_03 , released September 17, 2026
Allele: Dmel\pleFStrunc.UAS
Open Close
General Information
Symbol
Dmel\pleFStrunc.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0249337
Feature type
allele
Associated gene
Dmel\ple
Associated Insertion(s)
Carried in Construct
P{UAS-DTHgFS+}
Key Links
Transgenic product class
frameshift_variant
(Riemensperger et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
frameshift_variant
(Riemensperger et al., 2011)
Mutagen
in vitro construct
(Riemensperger et al., 2011)
Progenitor genotype
pleUAS.cFa
(Riemensperger et al., 2011)
Carried in construct
P{UAS-DTHgFS+}
Cytology
Description

UASt regulatory sequences are fused upstream of a genomic fragment that encodes the entire ple transcription unit. A frameshift mutation (single base insertion) has been introduced near the end of exon d (an exon that is specific to the non-neural isoform). This transgene is expected to express a full-length active protein in neural tissues, but in non-neural tissues, incorporation of exon d (containing the frameshift) is expected to result in a protein that is truncated before the catalytic domain, rendering it inactive.

(Riemensperger et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
ple
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4ple.PF, pleFStrunc.UAS, Scer\GAL4Ddc.PL fails to rescue ple2

      (Riemensperger et al., 2011)
      Comments

      Expression of pleFStrunc.Scer\UAS under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL fails to rescue the embryonic lethality normally found in ple2 mutants.

      (Riemensperger et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      pleFStrunc.Scer\UAS
      pleFStrunc.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)