FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\HTT16Q.FL.UAS
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General Information
Symbol
Hsap\HTT16Q.FL.UAS
Species
H. sapiens
Name
FlyBase ID
FBal0249416
Feature type
allele
Associated gene
Hsap\HTT
Associated Insertion(s)
Carried in Construct
P{UAS-HTT.16Q.FL}
Also Known As
UAS-Htt.16Q.FL, UAS-HTT16Q
Key Links
Transgenic product class
wild_type
(Romero et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Romero et al., 2008)
Mutagen
in vitro construct
(Romero et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-HTT.16Q.FL}
Cytology
Description

UASt drives expression of the entire 3144 amino acid Hsap\HTT protein with a wild type polyQ tract of 16 amino acids.

(Romero et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\HTT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\HTT16Q.FL.Scer\UAS under the control of Scer\GAL4elav.PU has no significant effect on nighttime sleep. A small, but significant increase in daytime sleep bout number is seen, but overall daytime rest and average daytime sleep bout length are normal.

      (Gonzales and Yin, 2010)

      Expression of Hsap\HD16Q.FL.Scer\UAS using the strong Scer\GAL4GMR.PF driver does not result in depigmentation or disorganization of ommatidia.

      Flies expressing Hsap\HD16Q.FL.Scer\UAS using Scer\GAL4C164 do not show impaired motor performance as a function of age, as measured by a flying assay.

      Aged flies expressing Hsap\HD16Q.FL.Scer\UAS using Scer\GAL4C164 do not show a neurodegenerative phenotype: motor neurons innervating the indirect flight muscles and neuromuscular junctions are normal.

      Excitatory junction potentials (EJPs) at neuromuscular junctions of third instar larvae expressing Hsap\HD16Q.FL.Scer\UAS using Scer\GAL4elav-C155 are similar to controls at 1.2 mM, 0.25 mM and 0.6 mM extracellular Ca[2+].

      Resting synaptic Ca[2+] levels at presynaptic terminals are elevated by ~2-fold in Hsap\HD16Q.FL.Scer\UAS, Scer\GAL4elav-C155 larvae.

      (Romero et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the htt98E2 homozygous mutant background induces prominent collapse of the thorax due to severe loss of the muscles below, these phenotypes are not observed in either the Hsap\MAPTScer\UAS.T:Avic\GFP expressing flies or the htt98E2 mutants alone and can be rescued by co-expression of Hsap\HTT16Q.FL.Scer\UAS.

      The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is partially suppressed by expression of Hsap\HTT16Q.FL.Scer\UAS under the Scer\GAL4arm.PS driver and further worsened by expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the mutant background. This worsened phenotype can in turn also be fully partially rescued by co-expression of Hsap\HTT16Q.FL.Scer\UAS.

      (Rui et al., 2015)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\HD16Q.FL.Scer\UAS
      Hsap\HTT16Q.FL.Scer\UAS
      Hsap\HTT16Q.FL.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (18)