A 989 base pair fragment from the flanking non-coding or intronic region of nAcRalpha-34E is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.
Thermogenetic activation (using TrpA1UAS.(B).cKa at 31[o]C) of Scer\GAL4GMR19G01 neurons in adults results in a reduced proboscis extension reflex in response to tarsal application of sucrose solution, compared to controls.