FB2021_02, released April 13, 2021

Allele: Scer\GAL4GMR47E12

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General Information
Symbol
Scer\GAL4GMR47E12
Species
S. cerevisiae
Name
FlyBase ID
FBal0252861
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GMR47E12-GAL4}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Pfeiffer et al., 2011.2.9)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{GMR47E12-GAL4}
      Cytology
      Nature of the lesion
      Statement
      Reference

      A 2012 base pair fragment from the flanking non-coding or intronic region of CG31646 is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

      (Pfeiffer et al., 2011.2.9)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      GAL4
      Associated Sequence Features
      GMR47E12
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval cholinergic lateral interneuron 1
      (Hasegawa et al., 2016)
      larval cholinergic lateral interneuron 2
      (Hasegawa et al., 2016)
      Additional Information
      Statement
      Reference

      Use of Scer\GAL80ChAT.3.3P restricts expression of a UAS target to the CLI1 and CLI2 neurons by inhibiting Scer\GAL4GMR47E12 expression in other interneurons. Use of Scer\GAL80tsh-GAL80 restricts expression of a UAS target to the CLI1 neurons by inhibiting Scer\GAL4GMR47E12 expression in other interneurons.

      (Hasegawa et al., 2016)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 660 nm (red) light) of a subset of Scer\GAL4GMR47E12 cells (expression restricted using Scer\GAL80ChAT.3.3P, or the combination of Scer\GAL80ChAT.3.3P and Scer\GAL80tsh-GAL80) halts forwards locomotion and significantly induces contraction of the abdomen, compared to controls. In the case of the subset of Scer\GAL4GMR47E12 cells activated when only Scer\GAL80ChAT.3.3P is also present, neuromere-specific activation results in segment-specific contraction of body wall muscles.

      (Hasegawa et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4GMR47E12
      Name Synonyms
      Secondary FlyBase IDs
        References (3)