FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4GMR65C07
Open Close
General Information
Symbol
Scer\GAL4GMR65C07
Species
S. cerevisiae
Name
FlyBase ID
FBal0254047
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GMR65C07-GAL4}
Also Known As
R65C07
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2011.2.9)
Progenitor genotype
Carried in construct
P{GMR65C07-GAL4}
Cytology
Description

A 2833 base pair fragment from the flanking non-coding or intronic region of Lkr is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR65C07
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      neuron | subset of adult brain
      (Cavey et al., 2016)
      neuron | subset of adult pars intercerebralis
      (Cavey et al., 2016)
      neuron | subset of ellipsoid body
      (Cavey et al., 2016)
      neuron | subset of fan-shaped body
      (Cavey et al., 2016)
      neuron | subset of adult lateral horn
      (Cavey et al., 2016)
      neuron | subset of adult gnathal ganglion
      (Cavey et al., 2016)
      Additional Information
      Statement
      Reference

      ScerGAL4GMR65C07 drives expression in a dense and complex mesh of neurons in the adult brain. A subset of these neurons project to or have cell bodies in the adult pars intercerebralis, adult gnathal ganglion, ellipsoid body, lateral horn and fan-shaped body. Neurons in the lateral horn arborize in the posterior part of the brain and dendrites overlap extensively with projections from Lk positive adult Leucokinin LHKL lateral horn neurons, while synapses are found in the posterior part of the brain, primarily the fan-shaped body.

      (Cavey et al., 2016)
       
      Marker for
      Reflects expression of
      Lkr
      (Cavey et al., 2016)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 28[o]C) of Scer\GAL4GMR65C07 neurons in adults results in a significant reduction in the strength of circadian locomotor rhythms, a decrease in sleep and an increase in locomotor activity levels, compared to controls; addition of Scer\GAL80elav.PS to further restrict activation prevents activation-induced locomotor activity defects.

      Inhibition (using shi1.UAS at 28[o]C) of Scer\GAL4GMR65C07 neurons in adults increases sleep and significantly reduces locomotor activity, compared to controls.

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4GMR65C07 neurons in adults results in a significant reduction in the strength of circadian locomotor rhythms, compared to controls.

      Blocking the molecular clock (using cycΔ.UAS.Tag:MYC) in Scer\GAL4GMR65C07 neurons in adults does not significantly affect the strength of circadian locomotor rhythms, compared to controls.

      (Cavey et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      LkrGAL4
      (Murphy et al., 2016)
      Scer\GAL4GMR65C07
      Name Synonyms
      Secondary FlyBase IDs
        References (5)