FlyBase Allele Report: Hsap\BACE1[UAS.VTR]

General Information

Symbol

Hsap\BACE1^UAS.VTR

Species

H. sapiens

Name

FlyBase ID

FBal0256669

Feature type

allele

Associated gene

Hsap\BACE1

Associated Insertion(s)

Carried in Construct

P{UAS-BACE1.VTR}

Key Links

Transgenic product class

wild_type

(Singh and Mahoney, 2011.2.17)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Singh and Mahoney, 2011.2.17)

Mutagen

in vitro construct

(Singh and Mahoney, 2011.2.17)

Progenitor genotype

Carried in construct

P{UAS-BACE1.VTR}

Cytology

Description

UAS drives expression of Hsap\BACE1.

(Singh and Mahoney, 2011.2.17)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\BACE1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis} has visible phenotype, suppressible by Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Other

Statement

Reference

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has visible phenotype

(Fernandez-Funez et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has ommatidium phenotype, suppressible | partially by Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has lens phenotype, suppressible | partially by Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has ommatidium phenotype, suppressible | partially by Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has lens phenotype, suppressible | partially by Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis} has ommatidium phenotype, suppressible by Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis} has lens phenotype, suppressible by Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Other

Statement

Reference

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has ommatidium phenotype

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has interommatidial bristle phenotype

(Fernandez-Funez et al., 2015)

Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU has lens phenotype

(Fernandez-Funez et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR} and Hsap\BACE1^Scer\UAS.VTR using Scer\GAL4^GMR.PU results in small and disorganized eyes compared with wild-type. At higher magnification, these eyes contain fused ommatidia, abnormally differentiated lenses and hairs, and broken lenses.

Compared with the co-expression of Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR} and Hsap\BACE1^Scer\UAS.VTR, flies co-expressing Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR}, Hsap\BACE1^Scer\UAS.VTR, and Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} using Scer\GAL4^GMR.PU exhibit larger and better-organized eyes with fewer fused ommatidia and no broken lenses.

Compared with the co-expression of Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR} and Hsap\BACE1^Scer\UAS.VTR, flies co-expressing Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR}, Hsap\BACE1^Scer\UAS.VTR, and Mus\scFv^{42.2.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} using Scer\GAL4^GMR.PU exhibit larger and better-organized eyes with fewer fused ommatidia and no broken lenses.

Co-expression of the combination of Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} and Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} suppresses the size defects and the disorganization of eyes resulting from the co-expression of Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR} and Hsap\BACE1^Scer\UAS.VTR using Scer\GAL4^GMR.PU.

(Fernandez-Funez et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33804