FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\EndoGIcwk
Open Close
General Information
Symbol
Dmel\EndoGIcwk
Species
D. melanogaster
Name
crosswalk
FlyBase ID
FBal0256911
Feature type
allele
Associated gene
Dmel\EndoGI
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(O'Keefe et al., 2011)
Progenitor genotype
EndoGIGScE6
(O'Keefe et al., 2011)
Cytology
Description

In addition to the amino acid replacement, the progenitor P{GSV1}EndoGIGScE6 insertion has been precisely excised from the mutant chromosome.

(O'Keefe et al., 2011)

Amino acid replacement: ?82term.

(O'Keefe et al., 2011)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:16,258,426..16,258,426 [+]
Nucleotide change:

C16258426T

Amino acid change:

Q82term | EndoGI-PA; Q82term | EndoGI-PB; Q82term | EndoGI-PC

Reported amino acid change:

?82term

Comment:

In addition to the amino acid replacement, the progenitor P{GSV1}EndoGIGScE6 insertion has been precisely excised from the mutant chromosome.

(O'Keefe et al., 2011)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous clones in the wing disc grow as well as their wild-type twin spot clones.

      Homozygous animals do not eclose at the expected frequency (there is a 70% reduction compared to heterozygotes).

      EndoGIcwk/Df(2L)RA5 larvae grow more slowly than controls. Adult escapers are small and strikingly uncoordinated. They show a number of phenotypes, including tremors, crossed legs, an inability to right themselves and held-out wings.

      Mutant embryos have motor axon defects. The most frequent and dramatic effect is on the intersegmental nerve b (ISNb) which fails to defasciculate from the ISN in 53% of hemisegments. The entire ISN nerve occasionally fails to exist the central nervous system. Defects in segmental nerve c (SNc) are seen in 39% of hemisegments and defects in the SNa are seen in 42% of hemisegments.

      Only 19% of mutant females can fly.

      (O'Keefe et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      EndoGIcwk has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Abl1/Abl[+]

      (O'Keefe et al., 2011)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      EndoGIcwk has larval intersegmental nerve branch ISNb of A1-7 phenotype, suppressible | partially by Abl1/Abl[+]

      (O'Keefe et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Abl1/+ partially rescues the ISNb defasiculation defects seen in homozygous EndoGIcwk embryos.

      (O'Keefe et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      EndoGIcwk is partially rescued by EndoGIUAS.cOa/Scer\GAL4elav.PLu

      (O'Keefe et al., 2011)
      Comments

      Expression of EndoGIScer\UAS.cOa under the control of Scer\GAL4elav.PLu significantly improves the coordination of EndoGIcwk animals; 73% of females tested can fly. In addition, the motor axon defects seen in EndoGIcwk embryos are also partially rescued.

      (O'Keefe et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      EndoGIcwk
      endoGIcwk
      (O'Keefe et al., 2011)
      Name Synonyms
      crosswalk
      (O'Keefe et al., 2011)
      Secondary FlyBase IDs
        References (1)