FlyBase Allele Report: Hsap\BCL2[UAS.cGa]

General Information

Symbol

Hsap\BCL2^UAS.cGa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct b of Gaumer

FlyBase ID

FBal0256958

Feature type

allele

Associated gene

Hsap\BCL2

Associated Insertion(s)

Carried in Construct

P{UAS-hBcl-2.G}

Also Known As

UAS-bcl-2

Key Links

Transgenic product class

cDNA

(Gaumer et al., 2000)

wild_type

(Gaumer et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Gaumer et al., 2000)

wild_type

(Gaumer et al., 2000)

Mutagen

in vitro construct

(Gaumer et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-hBcl-2.G}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\BCL2 coding sequences (a EcoR1-EcoR1 fragment of Hsap\BCL2 cDNA).

(Gaumer et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\BCL2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death, with Scer\GAL4^da.G32

(Gaumer et al., 2000)

partially lethal - majority die

(Gaumer et al., 2000)

partially lethal - majority die, with Scer\GAL4^da.G32

(Gaumer et al., 2000)

radiation resistant, with Scer\GAL4^da.G32

(Gaumer et al., 2000)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Gaumer et al., 2000)

ommatidium | increased number, with Scer\GAL4^GMR.PF

(Gaumer et al., 2000)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Gaumer et al., 2000)

pigment cell, with Scer\GAL4^GMR.PF

(Gaumer et al., 2000)

Detailed Description

Statement

Reference

Embryos expressing Hsap\BCL2^Scer\UAS.cGa ubiquitously under the control of Scer\GAL4^da.G32 fail to exhibit most of the cell death pattern normally seen during mid-embryonic development. Cell death appears particularly reduced in the lateral part of the cephalic region as well as in the ventral portion of the embryo during germ band retraction. The number of dying cells throughout the central nervous system is also reduced as the ventral nerve cord condenses.

Hsap\BCL2^Scer\UAS.cGa heterozygotes are sub-viable, for all lines tested. Upon induction with Scer\GAL4^da.G32, the percentage of lethality increases from 25% to 37%. Lethality occurs at earlier stages upon increasing the dose of Scer\GAL4^da.G32 or Hsap\BCL2^Scer\UAS.cGa, ranging from mostly larval death to embryonic death.

Ubiquitous expression of Hsap\BCL2^Scer\UAS.cGa (under the control of Scer\GAL4^da.G32 significantly reduced cell death induced by X-rays (4000 Rads) and accompanying levels of lethality.

Hsap\BCL2^Scer\UAS.cGa expression in the developing eye disc (under the control of Scer\GAL4^GMR.PF) leads to a rough eye phenotype in 45% of flies. This phenotype is enhanced and affects 100% of the flies expressing two doses of the Hsap\BCL2^Scer\UAS.cGa transgene under the control of Scer\GAL4^GMR.PF. These flies also exhibit supernumerary ommatidia and larger ommatidia, compared to wild-type. The retinal cell layer is disorganized compared to the regular pattern in wild-type. An increased spacing corresponding to additional cellular material between ommatidia is observed next to R1-R6 for each ommatidial unit. The position of this extra material correlates with the position where some of the pigment cells normally die by apoptosis in the pupal retina. A normal complement of photoreceptor cells is observed, indicating that Hsap\BCL2 alters the organisation of the eye by inhibiting apoptosis of pigment cells in the developing eye.

Expression of Hsap\BCL2^Scer\UAS.cGa in the wing disc under the control of Scer\GAL4^vg.PM has no detectable effect.

(Gaumer et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Hsap\BCL2^UAS.cGa, Scer\GAL4^da.G32 has decreased cell death phenotype, non-suppressible by Scer\GAL4^vg.PM

(Gaumer et al., 2000)

Suppressor of

Statement

Reference

Hsap\BCL2^UAS.cGa, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Hsap\DEK^UAS.cLa, Scer\GAL4^GMR.PU

(Lee et al., 2008)

Hsap\BCL2^UAS.cGa, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^GMR.PF

(Gaumer et al., 2000)

Hsap\BCL2^UAS.cGa/Scer\GAL4^GMR.PF is a suppressor of lethal phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Gaumer et al., 2000)

NOT Suppressor of

Statement

Reference

Hsap\BCL2^UAS.cGa, Scer\GAL4^vg.PM is a non-suppressor of lethal | pupal stage phenotype of Scer\GAL4^vg.PM, stwl^UY823

(Brun et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsap\BCL2^UAS.cGa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Hsap\DEK^UAS.cLa, Scer\GAL4^GMR.PU

(Lee et al., 2008)

Hsap\BCL2^UAS.cGa, Scer\GAL4^vg.PM is a suppressor of wing | temperature conditional phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Gaumer et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\BCL2^Scer\UAS.cGa partially suppresses the rough eye phenotype seen in flies expressing Hsap\DEK^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PU.

(Lee et al., 2008)

Ubiquitous co-expression of Hsap\BCL2^Scer\UAS.cGa and Mmus\Bax^Scer\UAS.cGb under the control of Scer\GAL4^GMR.PF results in a pattern of cell death similar to flies expressing Hsap\BCL2^Scer\UAS.cGa alone.

Expression of Hsap\BCL2^Scer\UAS.cGa is able to rescue the lethality of Mmus\Bax^Scer\UAS.cGb-expressing flies at 21[o]C (both under the control of Scer\GAL4^vg.PM).

Co-expression of Hsap\BCL2^Scer\UAS.cGa in Mmus\Bax^Scer\UAS.cGb-expressing flies at 18[o]C induces a decrease in the ratio of strong wing phenotypes as opposed to intermediate and weak phenotypes and an increase of the ratio of weak wing phenotypes as opposed to intermediate and strong phenotypes. No significant effect was observed at 21 and 25[o]C. The structure of the wing is partially restored in these double mutants.

(Gaumer et al., 2000)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Hsap\BCL2^Scer\UAS.cGa

Hsap\BCL2^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct b of Gaumer

Saccharyomyces cerevisiae UAS construct b of Gaumer

Secondary FlyBase IDs

References (6)

Report Sections

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