FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\TrpA1UAS.mCherry
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General Information
Symbol
Dmel\TrpA1UAS.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0256973
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Carried in construct
Cytology
Description

The FRT cassette containing transcriptional stop sequences has been excised, allowing the UAS regulatory sequences to drive expression of the TrpA1 coding region tagged with mCherry.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Thermogenetic activation of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4Tk and Ecol\lexAfru.P1.T:Hsim\VP16) increases male-male aggression compared to controls. Ecol\lexAfru.P1.T:Hsim\VP16 drives expression of Scer\FLP1L.8xEcol\lexAop, which results in excision of the Scer\FRT cassette from TrpA1Scer\FRT.stop.Scer\UAS.T:Disc\RFP-mCherry enabling these cells to express TrpA1 under the control of Scer\GAL4Tk when the flies are shifted to the permissive temperature. Activation of Tk-GAL4[Fru[M] neurons does not induce aggression of male flies towards females, and they copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
TrpA1Scer\UAS.T:Disc\RFP-mCherry
TrpA1UAS.mCherry
Name Synonyms
Secondary FlyBase IDs
    References (3)