FlyBase Allele Report: Dmel\Mi-2[HMS00301]

General Information

Symbol

Dmel\Mi-2^HMS00301

Species

D. melanogaster

Name

FlyBase ID

FBal0257196

Feature type

allele

Associated gene

Dmel\Mi-2

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00301}

Also Known As

HMS00301, Mi-2 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00301}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00301

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^fl.SCA3.UAS

(Ashraf et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

lethal, with Scer\GAL4^en.PU

(Dietz et al., 2015)

partially lethal - majority die, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

short lived, with Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

visible, with Scer\GAL4^bbg-C96

(Ellis et al., 2015)

visible | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

wing, with Scer\GAL4^bbg-C96

(Ellis et al., 2015)

wing, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Detailed Description

Statement

Reference

Expression of Mi-2^HMS00301 is pupal lethal with Scer\GAL4^ey.PH (escapers display strong eye phenotype, same result observed at 18[o]C) as well as Scer\GAL4^en.PU (same at 18[o]C) and results in small unfurled wings with many stuck in pupal cases (with Scer\GAL4^nub.PK). Scer\GAL4^Act5C.PI-driven expression is viable; no overt adult phenotype is observed with Scer\GAL4^GMR.PF.

(Dietz et al., 2015)

Expression of Mi-2^HMS00301 under the control of Scer\GAL4^bbg-C96 results in wing curling and a subset of wings exhibit nicking.

(Ellis et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Mi-2^HMS00301, Scer\GAL4^Switch1.106 has short lived phenotype, non-enhanceable by foxo^UAS.cFa, Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

NOT suppressed by

Statement

Reference

Mi-2^HMS00301, Scer\GAL4^Switch1.106 has short lived phenotype, non-suppressible by foxo^UAS.cFa, Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Enhancer of

Statement

Reference

Mi-2^HMS00301, Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Ellis et al., 2015)

Other

Statement

Reference

Mi-2^HMS00301, Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS has partially lethal - majority die phenotype

(Ellis et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Mi-2^HMS00301, Scer\GAL4^bbg-C96 is an enhancer of wing phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Ellis et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Mi-2^HMS00301 enhances the wing nicking phenotype of flies expressing dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96, and also leads to partial lethality.

(Ellis et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33419