FlyBase Allele Report: Dmel\aPKC[HMS01320]

General Information

Symbol

Dmel\aPKC^HMS01320

Species

D. melanogaster

Name

FlyBase ID

FBal0257913

Feature type

allele

Associated gene

Dmel\aPKC

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01320}

Also Known As

aPKC RNAi, HMS01320, UAS-aPKC-RNAi, UAS-aPKC RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01320}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01320

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing aPKC^HMS01320 under the control of Scer\GAL4^wor.PA leads to defects in cell polarity in larval neuroblasts.

(Hannaford et al., 2019)

The expression of aPKC^HMS01320 under the control of Scer\GAL4^insc-Mz1407 leads to type II neuroblasts in the third instar larval brain abnormally exhibiting symmetric cell divisions, and presenting significant decreases in cell size and number per brain lobe, as compared to controls.

(Liu et al., 2017)

Embryos from mothers expressing aPKC^HMS01320 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{otu.T:Hsim\VP16} or under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} display abnormal centrosome positioning in the embryonic epithelium.

(Jiang et al., 2015)

Females heterozygous for aPKC^HMS01320 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to aPKC^HMS01320 males results in: "few eggs,28% hatching"; maternal effect.

(Sopko et al., 2014)

Testes of males expressing aPKC^HMS01320 under the control of the triple "MTD" driver line (Scer\GAL4^nos.PG, Scer\GAL4^{nos.UTR.T:Hsim\VP16} and Scer\GAL4^{otu.T:Hsim\VP16}) sometimes contain misoriented spermatid cysts where the clustered spermatid nuclei are found apically (rather than basally).

(Xu et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, aPKC^HMS01320 has decreased cell number | third instar larval stage phenotype, suppressible by lilli^{UAS.SR.Tag:FLAG}/Scer\GAL4^insc-Mz1407/Su(Tpl)^{UAS.SR.Tag:MYC}

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, aPKC^HMS01320 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by lilli^{UAS.SR.Tag:FLAG}/Scer\GAL4^insc-Mz1407/Su(Tpl)^{UAS.SR.Tag:MYC}

(Liu et al., 2017)

NOT Suppressor of

Statement

Reference

aPKC^HMS01320/Scer\GAL4^αTub84B.PL is a non-suppressor of increased cell number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, aPKC^HMS01320 has type II neuroblast | third instar larval stage phenotype, suppressible by lilli^{UAS.SR.Tag:FLAG}/Scer\GAL4^insc-Mz1407/Su(Tpl)^{UAS.SR.Tag:MYC}

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, aPKC^HMS01320 has larval brain | third instar larval stage phenotype, suppressible by lilli^{UAS.SR.Tag:FLAG}/Scer\GAL4^insc-Mz1407/Su(Tpl)^{UAS.SR.Tag:MYC}

(Liu et al., 2017)

Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG, aPKC^HMS01320 has centrosome | maternal effect | embryonic stage phenotype, suppressible | maternal effect by baz^RNAi.UASp/Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Jiang et al., 2015)

Scer\GAL4^{VP16.mat.αTub67C}, aPKC^HMS01320 has centrosome | maternal effect | embryonic stage phenotype, suppressible | maternal effect by Dhc64C^GL00543, Scer\GAL4^{VP16.mat.αTub67C}

(Jiang et al., 2015)

Scer\GAL4^{VP16.mat.αTub67C}, aPKC^HMS01320 has centrosome | maternal effect | embryonic stage phenotype, suppressible | maternal effect by Dhc64C^HMS01587, Scer\GAL4^{VP16.mat.αTub67C}

(Jiang et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, aPKC^HMS01320 has centrosome | maternal effect | embryonic stage phenotype, non-suppressible | maternal effect by baz^{S980E.UASp.mGFP6}/Scer\GAL4^{VP16.mat.αTub67C}

(Jiang et al., 2015)

Scer\GAL4^{VP16.mat.αTub67C}, aPKC^HMS01320 has centrosome | maternal effect | embryonic stage phenotype, non-suppressible | maternal effect by baz^C.UASp.EGFP/Scer\GAL4^{VP16.mat.αTub67C}

(Jiang et al., 2015)

Scer\GAL4^{VP16.mat.αTub67C}, aPKC^HMS01320 has centrosome | maternal effect | embryonic stage phenotype, non-suppressible | maternal effect by par-1^W3/par-1[+]

(Jiang et al., 2015)

NOT Suppressor of

Statement

Reference

aPKC^HMS01320/Scer\GAL4^αTub84B.PL is a non-suppressor of interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number of type II neuroblasts in third instar larval brain lobes resulting from the expression of aPKC^HMS01320 under the control of Scer\GAL4^insc-Mz1407 is fully suppressed by the combined co-expression at low levels of Su(Tpl)^{Scer\UAS.SR.T:Hsap\MYC} and lilli^{Scer\UAS.SR.T:Zzzz\FLAG}.

(Liu et al., 2017)

Co-expression of baz^{dsRNA.shRNA.Scer\UAS.P\T} rescues the abnormal centrosome positioning of embryos from mothers expressing aPKC^HMS01320 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{otu.T:Hsim\VP16}.

par-1^W3/+, or co-expression of Dhc64C^GL00543, Dhc64C^HMS01587, but not baz^{S980E.Scer\UAS.P\T.T:Avic\GFP-m6} or baz^{C.Scer\UAS.P\T.T:Avic\GFP-EGFP}, rescues the abnormal centrosome positioning of embryos from mothers expressing aPKC^HMS01320 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.