FlyBase Allele Report: Scer\GAL4[sns.PK]

General Information

Symbol

Scer\GAL4^sns.PK

Species

S. cerevisiae

Name

sticks and stones promoter construct of Kocherlakota

FlyBase ID

FBal0230396

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{sns-GAL4.K}

Also Known As

sns-Gal4, snsGal4, sns::GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Kocherlakota et al., 2008)

Progenitor genotype

Carried in construct

P{sns-GAL4.K}

Cytology

Description

A 5kb fragment including 2kb upstream of the sns genomic region regulates expression of a GAL4 driver.

(Kocherlakota et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

sns

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 15

embryonic garland cell

(Zhuang et al., 2009)

embryonic stage 13 -- 16

fusion competent cell

(Haralalka et al., 2014)

embryonic stage 15 -- 16

fusion competent cell

(Shelton et al., 2009)

embryonic stage 15 -- third instar larval stage

embryonic/larval garland cell

(Zhuang et al., 2009)

pupal stage

fusion competent cell

(Brunetti et al., 2015)

Additional Information

Statement

Reference

Drives expression in fusion competent cells in embryos and pupae.

(FlyBase Curators, 2017)

ScerGAL4^sns.PK drives expression in pupal fusion competent myoblasts, but not in muscle founder cells.

(Brunetti et al., 2015)

ScerGAL4^sns.PK drives expression in fusion-competent myoblasts.

(Shelton et al., 2009)

Marker for

Reflects expression of

sns

(Brunetti et al., 2015, Shelton et al., 2009, Zhuang et al., 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | embryonic stage, with Kul^GD12729

(Jacobs, 2018.7.9)

lethal - all die during pharate adult stage, with Scer\GAL4^{kirre-rP298-G4}, sing^GD3396

(Brunetti et al., 2015)

viable, with sing^GD3396

(Brunetti et al., 2015)

Phenotype Manifest In

adult muscle system, with Scer\GAL4^{kirre-rP298-G4}, sing^GD3396

(Brunetti et al., 2015)

embryonic/larval garland cell, with sns^ZF1.4, sns^mIg4.UAS

(Zhuang et al., 2009)

embryonic/larval garland cell, with sns^ZF1.4, sns^mIg6.UAS

(Zhuang et al., 2009)

embryonic/larval salivary gland | embryonic stage, with FoxL1^UAS.cHa

(Hanlon and Andrew, 2016)

fusion competent cell, with dia^{ΔDad.UASp.EGFP}

(Haralalka et al., 2014)

garland cell, with aPKC^HMS01320

(Heiden et al., 2021)

garland cell, with baz^RNAi.UAS

(Heiden et al., 2021)

garland cell, with baz^{S980A.UAS.GFP}

(Heiden et al., 2021)

garland cell, with dlg1^HMS00172

(Heiden et al., 2021)

garland cell, with kirre^KK107679

(Heiden et al., 2021)

garland cell, with l(2)gl^KK100777

(Heiden et al., 2021)

garland cell, with Lkb1^HMS01351

(Heiden et al., 2021)

garland cell, with par-1^HMS00405

(Heiden et al., 2021)

garland cell, with par-6^GD4048

(Heiden et al., 2021)

garland cell, with scrib^HMS01993

(Heiden et al., 2021)

larval muscle system | embryonic stage, with barr^E831/Df(2L)Exel7077, barr^{UASp.cOa.EGFP}

(Jacobs, 2018.7.9)

larval muscle system | embryonic stage, with Kul^GD12729

(Jacobs, 2018.7.9)

nephrocyte, with Hsap\INF2^{L57P.UAS.Tag:MYC}

(Bayraktar et al., 2020)

nephrocyte, with Hsap\INF2^{L132R.UAS.Tag:MYC}

(Bayraktar et al., 2020)

nephrocyte, with Hsap\INF2^{S186P.UAS.Tag:MYC}

(Bayraktar et al., 2020)

nephrocyte, with Hsap\INF2^{ΔDID.UAS.Tag:MYC}

(Bayraktar et al., 2020)

nephrocyte diaphragm | larval stage, with Actn^GD1354

(Odenthal et al., 2023)

nephrocyte diaphragm | larval stage, with Actn^KK102286

(Odenthal et al., 2023)

slit diaphragm, with baz^{S980A.UAS.GFP}

(Heiden et al., 2021)

slit diaphragm | decreased number, with l(2)gl^KK100777

(Heiden et al., 2021)

slit diaphragm | decreased number, with par-1^HMS00405

(Heiden et al., 2021)

slit diaphragm | decreased number, with par-6^GD4048

(Heiden et al., 2021)

slit diaphragm | decreased number, with scrib^HMS01993

(Heiden et al., 2021)

visceral muscle fiber | embryonic stage, with Rac1^V12.UAS

(Liu et al., 2013)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Actn^KK102286, Scer\GAL4^sns.PK has nephrocyte diaphragm | larval stage phenotype, non-enhanceable by Hsap\ACTN4^{K255E.UAS.Tag:HA}, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Actn^KK102286, Scer\GAL4^sns.PK has nephrocyte diaphragm | larval stage phenotype, non-enhanceable by Hsap\ACTN4^{W59R.UAS.Tag:HA}, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Actn^KK102286, Scer\GAL4^sns.PK has nephrocyte diaphragm | larval stage phenotype, non-enhanceable by Hsap\ACTN4^{M240T.UAS.Tag:HA}, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Suppressed by

Statement

Reference

Actn^KK102286, Scer\GAL4^sns.PK has nephrocyte diaphragm | larval stage phenotype, suppressible | partially by Hsap\ACTN4^{UAS.WT.Tag:HA}, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Scer\GAL4^sns.PK, baz^RNAi.UAS has garland cell phenotype, suppressible by aPKC^{UAS.Tag:PH(PLCδ-Unk)}/Scer\GAL4^sns.PK

(Heiden et al., 2021)

NOT suppressed by

Statement

Reference

Actn^KK102286, Scer\GAL4^sns.PK has nephrocyte diaphragm | larval stage phenotype, non-suppressible by Hsap\ACTN4^{K255E.UAS.Tag:HA}, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Actn^KK102286, Scer\GAL4^sns.PK has nephrocyte diaphragm | larval stage phenotype, non-suppressible by Hsap\ACTN4^{W59R.UAS.Tag:HA}, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Actn^KK102286, Scer\GAL4^sns.PK has nephrocyte diaphragm | larval stage phenotype, non-suppressible by Hsap\ACTN4^{M240T.UAS.Tag:HA}, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Scer\GAL4^sns.PK, baz^RNAi.UAS has garland cell phenotype, non-suppressible by Scer\GAL4^sns.PK/aPKC^ΔN.UAS

(Heiden et al., 2021)

Scer\GAL4^sns.PK, baz^RNAi.UAS has garland cell phenotype, non-suppressible by aPKC^{UAS.Tag:CAAX(Unk)}/Scer\GAL4^sns.PK

(Heiden et al., 2021)

NOT Enhancer of

Statement

Reference

Hsap\ACTN4^{K255E.UAS.Tag:HA}, Scer\GAL4^sns.PK is a non-enhancer of nephrocyte diaphragm | larval stage phenotype of Actn^KK102286, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Hsap\ACTN4^{W59R.UAS.Tag:HA}, Scer\GAL4^sns.PK is a non-enhancer of nephrocyte diaphragm | larval stage phenotype of Actn^KK102286, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Hsap\ACTN4^{M240T.UAS.Tag:HA}, Scer\GAL4^sns.PK is a non-enhancer of nephrocyte diaphragm | larval stage phenotype of Actn^KK102286, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Suppressor of

Statement

Reference

Hsap\ACTN4^{UAS.WT.Tag:HA}, Scer\GAL4^sns.PK is a suppressor | partially of nephrocyte diaphragm | larval stage phenotype of Actn^KK102286, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

aPKC^{UAS.Tag:PH(PLCδ-Unk)}/Scer\GAL4^sns.PK is a suppressor of garland cell phenotype of Scer\GAL4^sns.PK, baz^RNAi.UAS

(Heiden et al., 2021)

Rac1^V12.UAS/Scer\GAL4^sns.PK is a suppressor of visceral muscle fiber | embryonic stage phenotype of msk⁴

(Liu et al., 2013)

Rac1^V12.UAS/Scer\GAL4^sns.PK is a suppressor of embryonic myoblast phenotype of mbc^D11.2

(Haralalka et al., 2011)

hbs::sns^{UAS.HESTC.Tag:HA}/Scer\GAL4^sns.PK is a suppressor of embryonic myoblast phenotype of sns^ZF1.4

(Shelton et al., 2009)

hbs::sns^{UAS.SETHC.Tag:HA}/Scer\GAL4^sns.PK is a suppressor of embryonic myoblast phenotype of sns^ZF1.4

(Shelton et al., 2009)

hbs::sns^{UAS.SEHTC.Tag:HA}/Scer\GAL4^sns.PK is a suppressor of embryonic myoblast phenotype of sns^ZF1.4

(Shelton et al., 2009)

Scer\GAL4^sns.PK/hbs^UAS.Tag:HA is a suppressor of embryonic myoblast phenotype of sns^ZF1.4

(Shelton et al., 2009)

NOT Suppressor of

Statement

Reference

Hsap\ACTN4^{M240T.UAS.Tag:HA}, Scer\GAL4^sns.PK is a non-suppressor of nephrocyte diaphragm | larval stage phenotype of Actn^KK102286, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Hsap\ACTN4^{K255E.UAS.Tag:HA}, Scer\GAL4^sns.PK is a non-suppressor of nephrocyte diaphragm | larval stage phenotype of Actn^KK102286, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Hsap\ACTN4^{W59R.UAS.Tag:HA}, Scer\GAL4^sns.PK is a non-suppressor of nephrocyte diaphragm | larval stage phenotype of Actn^KK102286, Scer\GAL4^sns.PK

(Odenthal et al., 2023)

Scer\GAL4^sns.PK/aPKC^ΔN.UAS is a non-suppressor of garland cell phenotype of Scer\GAL4^sns.PK, baz^RNAi.UAS

(Heiden et al., 2021)

aPKC^{UAS.Tag:CAAX(Unk)}/Scer\GAL4^sns.PK is a non-suppressor of garland cell phenotype of Scer\GAL4^sns.PK, baz^RNAi.UAS

(Heiden et al., 2021)

Scer\GAL4^sns.PK/Rac1^UAS.cLa is a non-suppressor of embryonic myoblast phenotype of mbc^D11.2

(Haralalka et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Pak3^UAS.Tag:V5/Scer\GAL4^sns.PK rescues Pak3^del

(Duan et al., 2012)

mbc^UAS.Tag:HA/Scer\GAL4^sns.PK rescues mbc^D11.2

(Haralalka et al., 2011)

mbc^{NPxxP.Δ1807.UAS.Tag:HA}/Scer\GAL4^sns.PK rescues mbc^D11.2

(Haralalka et al., 2011)

blow^UAS.cJa/Scer\GAL4^sns.PK rescues blow¹

(Jin et al., 2011)

Scer\GAL4^sns.PK/sns^UAS.Tag:HA rescues sns^ZF1.4

(Kocherlakota et al., 2008)

sns^{5xPDZ.UAS.Tag:HA}/Scer\GAL4^sns.PK rescues sns^ZF1.4

(Kocherlakota et al., 2008)

sns^{A17.UAS.Tag:HA}/Scer\GAL4^sns.PK rescues sns^ZF1.4

(Kocherlakota et al., 2008)

Partially rescues

sns^mIg4.UAS/Scer\GAL4^sns.PK partially rescues sns^ZF1.4

(Zhuang et al., 2009)

sns^mIg6.UAS/Scer\GAL4^sns.PK partially rescues sns^ZF1.4

(Zhuang et al., 2009)

sns^{F14.UAS.Tag:HA}/Scer\GAL4^sns.PK partially rescues sns^ZF1.4

(Kocherlakota et al., 2008)

sns^2xPXXP.UAS/Scer\GAL4^sns.PK partially rescues sns^ZF1.4

(Kocherlakota et al., 2008)

Fails to rescue

kirre^UAS.cRa/Scer\GAL4^sns.PK fails to rescue Df(1)w67k30

(Haralalka et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

92185

w¹¹¹⁸; P{sns-GAL4.K}6

92193

y¹ w¹¹¹⁸; P{sns-GAL4.K}6, sns^ZF1.4

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^sns.PK

Name Synonyms

sticks and stones promoter construct of Kocherlakota

Secondary FlyBase IDs

References (26)

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