The expression of Kul^GD12729 can sometimes produce embryos with a disarrayed muscle pattern and reduced muscle mass (Scer\GAL4^how-24B-driven expression), a relatively normal musculature, with some muscles appearing thinner (Scer\GAL4^twi.PB-driven expression) or some disoriented muscles and many thinner muscles, verging towards a "mini muscle" phenotype (Scer\GAL4^sns.PK-driven expression), as compared to controls.

Adults expressing Kul^GD12729 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.