FlyBase Allele Report: Dmel\msk[4]

General Information

Symbol

Dmel\msk⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0122949

Feature type

allele

Associated gene

Dmel\msk

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Baker et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: R314term.

(Baker et al., 2002)

Nucleotide substitution: C940T.

(Baker et al., 2002)

Amino acid replacement: ??term.

(Lorenzen et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,118,272..8,118,272 [+]

Nucleotide change:

C8118272T

Reported nucleotide change:

C940T

Amino acid change:

R314term | msk-PA

Reported amino acid change:

R314term

(Baker et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | embryonic stage | recessive

(Baker et al., 2002)

lethal | larval stage | recessive

(Baker et al., 2002)

lethal | recessive

(Lorenzen et al., 2001)

Phenotype Manifest In

visceral muscle fiber | embryonic stage

(Liu et al., 2013)

visceral muscle fiber | embryonic stage (with msk⁵)

(Liu et al., 2013)

Detailed Description

Statement

Reference

msk⁴/msk⁵ or msk⁴/msk⁴ embryos show a muscle detachment phenotype.

(Liu et al., 2013)

Homozygotes die as late embryos or early larvae, with a normal looking cuticle.

(Baker et al., 2002)

Lethality acts in the late embryo or early larval stages. Embryos are morphologically wild type.

(Lorenzen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

msk⁴ is an enhancer of visible phenotype of rho^Stg

(Baker et al., 2002)

msk⁴ is an enhancer of visible phenotype of rho^Wk

(Baker et al., 2002)

Suppressor of

Statement

Reference

msk⁴/msk[+] is a suppressor of visible phenotype of Scer\GAL4^c684, if^m8.UAS

(Baker et al., 2002)

msk⁴ is a suppressor of visible phenotype of if^m8.αTub84B

(Baker et al., 2002)

NOT Suppressor of

Statement

Reference

msk⁴ is a non-suppressor of visible phenotype of Egfr^E1

(Baker et al., 2002)

msk⁴ is a non-suppressor of visible phenotype of rl^Sem

(Baker et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

msk⁴/msk⁵ has visceral muscle fiber | embryonic stage phenotype, enhanceable by mbc^D11.2/mbc[+]

(Liu et al., 2013)

msk⁴/msk⁵ has visceral muscle fiber | embryonic stage phenotype, enhanceable by Ced-12^KO/Ced-12[+]

(Liu et al., 2013)

Suppressed by

Statement

Reference

msk⁴ has visceral muscle fiber | embryonic stage phenotype, suppressible by Scer\GAL4^Mef2.PR/Ced-12^UAS.cGa

(Liu et al., 2013)

msk⁴ has visceral muscle fiber | embryonic stage phenotype, suppressible by Scer\GAL4^Mef2.PR/mbc^UAS.cBa/Ced-12^UAS.cGa

(Liu et al., 2013)

msk⁴ has visceral muscle fiber | embryonic stage phenotype, suppressible by Scer\GAL4^Mef2.PR/Ced-12^EDE.UAS.EYFP

(Liu et al., 2013)

msk⁴ has visceral muscle fiber | embryonic stage phenotype, suppressible by Rac1^V12.UAS/Scer\GAL4^sns.PK

(Liu et al., 2013)

Enhancer of

Statement

Reference

msk⁴/msk[+] is an enhancer of visceral muscle fiber | embryonic stage phenotype of Ced-12^KO/Ced-12^19F3

(Liu et al., 2013)

msk⁴ is an enhancer of wing phenotype of rho^Stg

(Baker et al., 2002)

msk⁴ is an enhancer of wing vein | ectopic phenotype of rho^Wk

(Baker et al., 2002)

Suppressor of

Statement

Reference

msk⁴ is a suppressor of eye phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Pepple et al., 2007)

msk⁴ is a suppressor of wing phenotype of if^m8.αTub84B

(Baker et al., 2002)

msk⁴/msk[+] is a suppressor of wing phenotype of Scer\GAL4^c684, if^m8.UAS

(Baker et al., 2002)

NOT Suppressor of

Statement

Reference

msk⁴/msk[+] is a non-suppressor of visceral muscle fiber | embryonic stage phenotype of Ced-12^UAS.cGa, Scer\GAL4^Mef2.PR, mbc^UAS.cBa

(Liu et al., 2013)

msk⁴/msk[+] is a non-suppressor of tendon cell | embryonic stage phenotype of Ced-12^UAS.cGa, Scer\GAL4^Mef2.PR, mbc^UAS.cBa

(Liu et al., 2013)

msk⁴ is a non-suppressor of wing vein | ectopic phenotype of Egfr^E1

(Baker et al., 2002)

msk⁴ is a non-suppressor of wing vein | ectopic phenotype of rl^Sem

(Baker et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of msk⁴/+ increases the severity of muscle attachment defects in Ced-12^19F3/Ced-12^KO embryos.

The presence of mbc^D11.2/+ or Ced-12^KO/+ increases the severity of muscle attachment defects in msk⁴/msk⁵ embryos.

Co-expression of Ced-12^Scer\UAS.cGa and mbc^Scer\UAS.cBa via Scer\GAL4^Mef2.PR results in prevalent myoblast fusion, muscle attachment, and tendon cell identity phenotypes in embryos. The muscle attachment and tendon cell phenotypes are not rescued in a msk⁴/+ background.

Expression of Ced-12^Scer\UAS.cGa, Ced-12^{EDE.Scer\UAS.T:Avic\GFP-EYFP}, or Ced-12^Scer\UAS.cGa together with mbc^Scer\UAS.cBa via Scer\GAL4^Mef2.PR partially rescues the msk⁴ embryonic muscle detachment phenotype.

Expression of Rac1^V12.Scer\UAS via Scer\GAL4^sns.PK partially rescues the msk⁴ embryonic muscle detachment phenotype.

(Liu et al., 2013)

The wing blistering caused by expression of if^m8.αTub84B is suppressed by msk⁴/+. The wing blistering caused by expression of if^m8.Scer\UAS under the control of Scer\GAL4^337Y is suppressed by msk⁴/+.

(Baker et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a dominant suppressor of the wing blister phenotype caused by if^m8.Scer\UAS expressed under the control of Scer\GAL4^c684.

(Baker et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

msk⁴

(Liu et al., 2013, Pepple et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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