Co-expression of l(2)glKK100777 exacerbates the increased hemocyte count observed in larvae expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Hml.Δ; these larvae are viable up to pupal stage and prepare for pupation, but only a subset of these pupae successfully eclose at 25[o]C, as compared to controls. None of these flies eclose when reared at 29[o]C, and pupae exhibit dessication and an absence of precursors of adult structures, in contrast to controls.
Larvae expressing both Ras85DV12.Scer\UAS and l(2)glKK100777 under the control of Scer\GAL4Hml.Δ exhibit no significant difference in wound healing; larvae wounded and infected with the bacteria Staphylococcus aureus, Erwinia carotovora, or E.coli survive equally well as controls; and hemocytes isolated from larvae exhibit normal phagocytosis levels upon exposure to to heat killed E. coli, as compared to controls.
Larvae expressing both Ras85DV12.Scer\UAS and l(2)glKK100777 under the control of Scer\GAL4Bx-MS1096 infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens, do not show a significant difference in survival as compared to controls; but when these constructs are driven under the control of Scer\GAL4Hml.Δ, infected larvae show a significant increase in mortality as compared to controls, and also show a similar decrease in motility upon nematode infection as seen in those expressing only Ras85DV12.Scer\UAS.
The tumor-like overgrowth of MARCM clones in larval antennal disc expressing l(2)glKK100777 under the control of Scer\GAL4Dll-md23 is not significantly worsened by cnoR2 homozygosity but the cellular and tissue morphology and disorganization are exacerbated.