FlyBase Allele Report: Dmel\l(2)gl[KK100777]

General Information

Symbol

Dmel\l(2)gl^KK100777

Species

D. melanogaster

Name

FlyBase ID

FBal0258819

Feature type

allele

Associated gene

Dmel\l(2)gl

Associated Insertion(s)

Carried in Construct

P{KK100777}

Also Known As

UAS-l(2)gl^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100777}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100777

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of leukemia

CEA with Ras85D^V12.UAS

(Arefin et al., 2017)

model of brain cancer

CEA with Ras85D^V12.cUa.UAS

(Manzanero-Ortiz et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone, with Scer\GAL4^Dll-md23

(Rives-Quinto et al., 2017)

abnormal size | somatic clone | third instar larval stage, with Scer\GAL4^Dll-md23

(Rives-Quinto et al., 2017)

increased cell number | somatic clone | third instar larval stage, with Scer\GAL4^Dll-md23

(Rives-Quinto et al., 2017)

neoplasia | somatic clone | third instar larval stage, with Scer\GAL4^Dll-md23

(Rives-Quinto et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of l(2)gl^KK100777 under the control of Scer\GAL4^Bx-MS1096 or Scer\GAL4^Hml.Δ does not lead to a significant difference in survival as compared to controls when larvae are infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens.

(Arefin et al., 2017)

Expression of l(2)gl^KK100777 (driven by Scer\GAL4^Dll-md23) in MARCM clones in the antennal discs in third instar larvae leads to tumorigenic overgrowth, while in the type II neuroblast lineage clones it results in supernumerary neuroblasts but the clones are smaller than the wild-type control clones.

(Rives-Quinto et al., 2017)

Scer\GAL4^tj-NP1624-mediated expression results in the transformation of the normal germline cyst epithelial monolayer into two or more cell layers. Individual cells or cell clusters seem to ingress into the cyst.

(Berns et al., 2014)

Expression of l(2)gl^KK100777 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^repo.PU has no detectable effect on glial cell number in the third instar larval eye disc.

(Reddy and Irvine, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

l(2)gl^KK100777, Scer\GAL4^Hml.Δ is an enhancer of increased cell number | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.Δ

(Arefin et al., 2017)

NOT Enhancer of

Statement

Reference

l(2)gl^KK100777, Scer\GAL4^Hml.Δ is a non-enhancer of abnormal locomotor behavior | larval stage | conditional phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.Δ

(Arefin et al., 2017)

l(2)gl^KK100777/Scer\GAL4^Dll-md23 is a non-enhancer of abnormal neuroanatomy | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

l(2)gl^KK100777/Scer\GAL4^Dll-md23 is a non-enhancer of abnormal cell number | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

l(2)gl^KK100777/Scer\GAL4^Dll-md23 is a non-enhancer of decreased cell size | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

NOT Suppressor of

Statement

Reference

l(2)gl^KK100777, Scer\GAL4^Hml.Δ is a non-suppressor of abnormal locomotor behavior | larval stage | conditional phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.Δ

(Arefin et al., 2017)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Hml.Δ, l(2)gl^KK100777 has abnormal immune response | larval stage phenotype

(Arefin et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Hml.Δ, l(2)gl^KK100777 has lethal - all die before end of P-stage phenotype

(Arefin et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Dll-md23, l(2)gl^KK100777 has antennal disc | third instar larval stage | somatic clone phenotype, enhanceable | somatic clone by cno^R2/cno^R2

(Rives-Quinto et al., 2017)

Enhancer of

Statement

Reference

l(2)gl^KK100777, Scer\GAL4^Hml.Δ is an enhancer of embryonic/larval hemocyte | larval stage | increased number phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.Δ

(Arefin et al., 2017)

NOT Enhancer of

Statement

Reference

l(2)gl^KK100777/Scer\GAL4^Dll-md23 is a non-enhancer of type II neuroblast | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of l(2)gl^KK100777 exacerbates the increased hemocyte count observed in larvae expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Hml.Δ; these larvae are viable up to pupal stage and prepare for pupation, but only a subset of these pupae successfully eclose at 25[o]C, as compared to controls. None of these flies eclose when reared at 29[o]C, and pupae exhibit dessication and an absence of precursors of adult structures, in contrast to controls.

Larvae expressing both Ras85D^V12.Scer\UAS and l(2)gl^KK100777 under the control of Scer\GAL4^Hml.Δ exhibit no significant difference in wound healing; larvae wounded and infected with the bacteria Staphylococcus aureus, Erwinia carotovora, or E.coli survive equally well as controls; and hemocytes isolated from larvae exhibit normal phagocytosis levels upon exposure to to heat killed E. coli, as compared to controls.

Larvae expressing both Ras85D^V12.Scer\UAS and l(2)gl^KK100777 under the control of Scer\GAL4^Bx-MS1096 infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens, do not show a significant difference in survival as compared to controls; but when these constructs are driven under the control of Scer\GAL4^Hml.Δ, infected larvae show a significant increase in mortality as compared to controls, and also show a similar decrease in motility upon nematode infection as seen in those expressing only Ras85D^V12.Scer\UAS.

(Arefin et al., 2017)

The tumor-like overgrowth of MARCM clones in larval antennal disc expressing l(2)gl^KK100777 under the control of Scer\GAL4^Dll-md23 is not significantly worsened by cno^R2 homozygosity but the cellular and tissue morphology and disorganization are exacerbated.

The decreased size of neuroblasts in the cno^R2 type II neuroblast lineage MARCM clones is not affected by expression of l(2)gl^KK100777 (driven by Scer\GAL4^Dll-md23) in the clones.

(Rives-Quinto et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v109604

P{KK100777}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

l(2)gl^KK100777

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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