FlyBase Allele Report: Dmel\Uba5[HMS01352]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\Uba5^HMS01352

General Information

Symbol

Dmel\Uba5^HMS01352

Species

D. melanogaster

Name

FlyBase ID

FBal0257945

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01352}

Key Links

Genomic Maps

Transgenic product class

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01352}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autosomal recessive spinocerebellar ataxia 24

CEC

(Duan et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

type I bouton | third instar larval stage, with Scer\GAL4^elav.PU

(Duan et al., 2016)

type I bouton | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Duan et al., 2016)

wing, with Scer\GAL4^da.PU

(Duan et al., 2016)

Detailed Description

Statement

Reference

Around 40% of flies with expression of Uba5^HMS01352 driven by Scer\GAL4^da.PU exhibit abnormal vertical-turned wings that fail to fold, and mutants show significant decreases in climbing ability and flight as well as shorter lifespans, compared to controls. Flies with expression of Uba5^HMS01352 driven by Scer\GAL4^da.PU do not show disrupted muscle morphology in the indirect flight muscles.

Third instar larvae with expression of Uba5^HMS01352 driven by Scer\GAL4^RapGAP1-OK6 or Scer\GAL4^elav.PU (but not Scer\GAL4^Mhc.PU) show significant decreases in type Ib bouton number and increases in bouton size at the neuromuscular junction, compared to controls.

(Duan et al., 2016)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, Uba5^HMS01352 has decreased rate of adult locomotory behavior | progressive phenotype, suppressible | partially by Atg9^UAS.GFP, Scer\GAL4^elav.PU

(Li et al., 2023)

Scer\GAL4^elav.PU, Uba5^HMS01352 has short lived phenotype, suppressible | partially by Atg9^UAS.GFP, Scer\GAL4^elav.PU

(Li et al., 2023)

Scer\GAL4^da.PU, Uba5^HMS01352 has visible phenotype, suppressible | partially by Hsap\UBA5^UAS.Tag:HA, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^da.PU, Uba5^HMS01352 has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Hsap\UBA5^UAS.Tag:HA, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^da.PU, Uba5^HMS01352 has visible phenotype, suppressible | partially by Hsap\UBA5^{R246X.UAS.Tag:HA}, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^da.PU, Uba5^HMS01352 has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Hsap\UBA5^{R246X.UAS.Tag:HA}, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^RapGAP1-OK6, Uba5^HMS01352 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Hsap\UBA5^UAS.Tag:HA, Scer\GAL4^RapGAP1-OK6

(Duan et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.PU, Uba5^HMS01352 has visible phenotype, non-suppressible by Hsap\UBA5^{K310E.UAS.Tag:HA}, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^da.PU, Uba5^HMS01352 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Hsap\UBA5^{K310E.UAS.Tag:HA}, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^RapGAP1-OK6, Uba5^HMS01352 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Hsap\UBA5^{R246X.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Duan et al., 2016)

Scer\GAL4^RapGAP1-OK6, Uba5^HMS01352 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Hsap\UBA5^{K310E.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Duan et al., 2016)

Enhancer of

Statement

Reference

Ufl1^HMJ30245, Uba5^HMS01352, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Tsc1^GD11836

(Li et al., 2023)

Ufl1^HMJ30245, Uba5^HMS01352, Scer\GAL4^GMR.PU is an enhancer of increased size | adult stage phenotype of Scer\GAL4^GMR.PU, Tsc1^GD11836

(Li et al., 2023)

Other

Statement

Reference

Scer\GAL4^elav.PU, Uba5^HMS01352, Ufl1^HMJ30245 has short lived phenotype

(Li et al., 2023)

Scer\GAL4^elav.PU, Uba5^HMS01352, Ufl1^HMJ30245 has increased cell death | adult stage phenotype

(Li et al., 2023)

Scer\GAL4^elav.PU, Uba5^HMS01352, Ufl1^HMJ30245 has abnormal oxidative stress response | adult stage phenotype

(Li et al., 2023)

Scer\GAL4^elav.PU, Uba5^HMS01352, Ufl1^HMJ30245 has decreased rate of adult locomotory behavior | progressive phenotype

(Li et al., 2023)

Scer\GAL4^elav.PU, Uba5^HMS01352, Ufl1^HMJ30245 has increased mortality | adult stage | chemical conditional phenotype

(Li et al., 2023)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^da.PU, Uba5^HMS01352 has wing phenotype, suppressible | partially by Hsap\UBA5^UAS.Tag:HA, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^da.PU, Uba5^HMS01352 has wing phenotype, suppressible | partially by Hsap\UBA5^{R246X.UAS.Tag:HA}, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^RapGAP1-OK6, Uba5^HMS01352 has type I bouton | third instar larval stage phenotype, suppressible by Hsap\UBA5^UAS.Tag:HA, Scer\GAL4^RapGAP1-OK6

(Duan et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.PU, Uba5^HMS01352 has wing phenotype, non-suppressible by Hsap\UBA5^{K310E.UAS.Tag:HA}, Scer\GAL4^da.PU

(Duan et al., 2016)

Scer\GAL4^RapGAP1-OK6, Uba5^HMS01352 has type I bouton | third instar larval stage phenotype, non-suppressible by Hsap\UBA5^{R246X.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Duan et al., 2016)

Scer\GAL4^RapGAP1-OK6, Uba5^HMS01352 has type I bouton | third instar larval stage phenotype, non-suppressible by Hsap\UBA5^{K310E.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Duan et al., 2016)

Enhancer of

Statement

Reference

Ufl1^HMJ30245, Uba5^HMS01352, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, Tsc1^GD11836

(Li et al., 2023)

Other

Statement

Reference

Scer\GAL4^elav.PU, Uba5^HMS01352, Ufl1^HMJ30245 has adult brain phenotype

(Li et al., 2023)

Scer\GAL4^elav.PU, Uba5^HMS01352, Ufl1^HMJ30245 has lysosome | increased number | adult stage phenotype

(Li et al., 2023)

Scer\GAL4^elav.PU, Uba5^HMS01352, Ufl1^HMJ30245 has vacuole | increased number | adult stage phenotype

(Li et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\UBA5^{Scer\UAS.T:Ivir\HA1} or Hsap\UBA5^{R246X.Scer\UAS.T:Ivir\HA1} (but not Hsap\UBA5^{K310E.Scer\UAS.T:Ivir\HA1}) partially rescues abnormal wing phenotypes and locomotor defects (increased climbing time) in flies with expression of Uba5^HMS01352 driven by Scer\GAL4^da.PU.

Co-expression of Hsap\UBA5^{Scer\UAS.T:Ivir\HA1} (but not Hsap\UBA5^{R246X.Scer\UAS.T:Ivir\HA1} or Hsap\UBA5^{K310E.Scer\UAS.T:Ivir\HA1}) rescues abnormal bouton number and size at the NMJ of larvae with expression of Uba5^HMS01352 driven by Scer\GAL4^RapGAP1-OK6.

(Duan et al., 2016)

Complementation and Rescue Data

Rescued by

Uba5^HMS01352 is rescued by Uba5^UAS.Tag:HA/Scer\GAL4^RapGAP1-OK6

(Duan et al., 2016)

Partially rescued by

Uba5^HMS01352 is partially rescued by Uba5^UAS.Tag:HA/Scer\GAL4^da.PU

(Duan et al., 2016)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

34363

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01352}attP2

HMS01352

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01352}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG1749^HMS01352

Uba5^HMS01352

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections