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General Information
Symbol
Dmel\mtmUAS.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0258482
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-mCherry:mtm
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a full-length mtm cDNA which is tagged with mCherry.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Primary macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA have an excess number of F-actin rich protrusions compared to wild type.

Primary hemocytes dissected from wild-type larvae undergo remodelling from a round to a spread cell shape, having F-actin rich radial protrusions. Primary hemocytes derived larvae expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA have an excessive number of these protrusions compared to wild-type controls.

Third instar larvae expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Pxn.PS have a normal number of bled hemocytes per larva and have a normal number of sessile hemocytes per segment, although the sessile hemocytes have an abnormally dispersed distribution compared to controls.

Third instar larvae expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Pxn.PS have a reduced number of hemocytes recruited to a puncture wound site 6 hours after wounding compared to control larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4He.PZ rescues the increased endolysosomal compartment phenotype of hemo-A4/hemo-A4 mutant hemocytes.

Expression of SbfGD12081 suppresses the increase in F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA. The macrophages completely lack protrusions, similar to what is seen when SbfGD12081 is expressed alone.

Expression of Pi3K68DGD7348 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

Expression of Rab21T27N.Scer\UAS.T:Avic\GFP suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

Expression of Rab21GD9042 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

Expression of Rab11KK108297 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of mtmdsRNA.cVa.Scer\UAS rescues the extra protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
mtmScer\UAS.T:Disc\RFP-mCherry
mtmUAS.mCherry
Name Synonyms
Secondary FlyBase IDs
    References (3)