FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\zld294
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General Information
Symbol
Dmel\zld294
Species
D. melanogaster
Name
FlyBase ID
FBal0260648
Feature type
allele
Associated gene
Dmel\zld
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Liang et al., 2008)
Mutagen
Delta2-3 transposase
(Liang et al., 2008)
Progenitor genotype
P{RS3}UM-8171-3
(Liang et al., 2008)
Cytology
Description

Generated by imprecise excision of P{RS3}UM-8171-3, resulting in a deletion of genomic sequence from -1270 to +1375 bp relative to the transcription start site of transcript vfl-RB.

(Liang et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:19,781,552..19,784,197 [-]
Comment:

Deletion resulting from imprecise excision of P{RS3}UM-8171-3. Reported as a deletion from -1270 to +1375 relative to the zld transcription start site (defined by the start site of zld-RB in earlier FlyBase releases, e.g. r5.1).

(Liang et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      zld294 heterozygous as well as zld294/zldZnF1 or zld294/zldEDD transheterozygous females are viable at reduced levels but zld294 hemizygous males are lethal. zld294/zldZnF2 and zld294/zldJAZ females are nearly completely lethal.

      (Hamm et al., 2017)

      Embryos derive from homozygous female germline clones do not cellularise and have no zygotically controlled mitosis corresponding to mitosis 14 in wild-type embryos.

      (Sung et al., 2013)

      Hemizygous embryos show abnormal central nervous system and head development.

      Embryos derived from vfl294 germ-line clones (that lack both maternal and zygotic vfl) show a severely abnormal morphology after mitotic cleavage cycle 14 and do not survive to make cuticle.

      Before mitotic cycle 14, embryos derived from vfl294 germ-line clones are similar to wild type except for sporadic nuclear fallout. At early cycle 14 , the hexagonal actin network becomes disorganized and begins to degenerate resulting in a multinucleated phenotype. Cellularization does not proceed as furrow canals never move inward. In addition, nuclei do not elongate but instead become rounded, enlarged and clumped together - regions of higher nuclear density are observed.

      (Liang et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos derived from double mutant vfl294 RpII215X161 female germline clones undergo at least 13 divisions.

      (Sung et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      zld294 is rescued by zldotu.PL

      (Liang et al., 2008)
      Comments

      Expression of vflotu.PL rescues the cellularization defects seen in embryos derived from vfl294 germ-line clones.

      (Liang et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (11)