nos^ΔBX, nos^BN/nos^BN females that are also heterozygous for wah¹ give rise to embryos that rarely hatch, concomitant with a reduction in the average number of abdominal segments from 3.7 to 1.9.

Small homozygous wah¹ clones in third instar salivary glands do not show any defects in proliferation, survival or endo-reduplication.

Germline clones of wah¹ arrest in early oogenesis.

wah¹ nos^BN/nos^BN females appear to be significantly less fertile than nos^BN/nos^BN females.

In contrast to nos^BN homozygous ovaries, ovaries from wah¹ nos^BN/nos^BN females have few normal ovarioles: many contain either no maturing egg chambers or only a single mature stage 13/14 oocyte; and many contain aberrant, small egg chambers that contain only one or two highly polyploid germ line nuclei.

In contrast to nos^BN/nos^BN females, wah¹, nos^BN/nos^BN females produce eggs for only 5-6 days after eclosion.

wah¹, nos^BN/nos^L7 females do not exhibit age-dependent loss of fecundity.

A single copy of wah^+t9.9 can rescue the age-dependent female infertility of wah¹, nos^BN/nos^BN females. However, wah¹, nos^BN homozygotes expressing a single copy of wah^+t9.9 exhibit age-dependent infertility, producing no eggs after the 8th day post-eclosion.

In ovarioles from very young wah¹, nos^BN/nos^BN flies, the spectrosomes of germline stem cells and cystoblasts and the fusomes of immature cysts appear normal. But slightly older ovaries are bereft of germline cells: some germaria contain only one or two germline cysts and many have none. In these older ovaries, differentiation or development of the few remaining cysts is abnormal, many of which contain one or two large polyploid cells.