FB2026_02 , released June 18, 2026
Allele: Dmel\painUAS.cSa
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General Information
Symbol
Dmel\painUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Senatore
FlyBase ID
FBal0260852
Feature type
allele
Associated gene
Dmel\pain
Associated Insertion(s)
Carried in Construct
P{UAS-pain.FL}
Key Links
Transgenic product class
cDNA
(Sénatore et al., 2011)
wild_type
(Sénatore et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Sénatore et al., 2011)
wild_type
(Sénatore et al., 2011)
Mutagen
in vitro construct
(Sénatore et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-pain.FL}
Cytology
Description

UAS regulatory sequences drive expression of pain. The full-length pain cDNA RE03641 was used a template for the pain sequence. This cDNA was found to contain a TN10 transposon following nucleotide 1795. The transposon sequence was removed using a two step PCR strategy. The PCR product was verified and found to contain a single silent mutation at nucleotide 1605 changing C into T. This PCR product was cloned into the vector and is expressed under the control of UAS regulatory sequences.

(Sénatore et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
pain
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      painUAS.cSa/Scer\GAL4unspecified rescues painEP2462

      (Sénatore et al., 2010)

      painUAS.cSa/Scer\GAL4NP1029 rescues painEP2462

      (Sénatore et al., 2010)
      Comments

      Expression of painScer\UAS.cSa in the heart under the control of Scer\GAL4unspecified rescues cardiac pauses during motion in painEP2462 mutant larvae.

      Expression of painScer\UAS.cSa under the control of Scer\GAL4NP1029 restores basal cardiac activity to wild-type levels in painEP2462 mutant larvae.

      (Sénatore et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      painScer\UAS.cSa
      painUAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Senatore
      Secondary FlyBase IDs
        References (2)