FlyBase Allele Report: Dmel\G9a[DD1]

General Information

Symbol

Dmel\G9a^DD1

Species

D. melanogaster

Name

FlyBase ID

FBal0260881

Feature type

allele

Associated gene

Dmel\G9a

Associated Insertion(s)

Carried in Construct

Also Known As

EHMT^DD1

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Kramer et al., 2011)

Mutagen

Delta2-3 transposase

(Kramer et al., 2011)

Progenitor genotype

P{SUPor-P}G9a^KG01242

(Kramer et al., 2011)

Cytology

Description

Imperfect excision of P{SUPor-P}G9a^KG01242, resulting in loss of 870 bp of DNA downstream of the insertion site which includes the translational start site.

(Kramer et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:246,142..247,011 [+]

Comment:

870 bp deletion resulting from the imprecise excsion of P{SUPor-P}G9a^KG01242 removes the G9a translation start site.

(Kramer et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Kleefstra syndrome 1

CEA

(Kramer et al., 2011)

CEA with G9a^DD2

(Kramer et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | adult stage

(Kramer et al., 2011)

abnormal learning | adult stage (with G9a^DD2)

(Kramer et al., 2011)

abnormal locomotor behavior | early third instar larval stage

(Kramer et al., 2011)

abnormal memory | adult stage

(Kramer et al., 2011)

abnormal neuroanatomy | third instar larval stage

(Kramer et al., 2011)

viable

(Kramer et al., 2011)

Phenotype Manifest In

larval ventral cluster group multidendritic neuron

(Kramer et al., 2011)

multidendritic dendrite | third instar larval stage

(Kramer et al., 2011)

Detailed Description

Statement

Reference

General nervous system development and neuronal function is not affected in mutant flies.

Dendrite development in multidendrite (md) neurons is altered in mutant flies. While the basic organization of dendritic arbours in type 4 md neurons is maintained, there is a reduction of higher order branching resulting in dendritic fields of appreciably reduced complexity. The total number of dendrite ends is significantly reduced.

The total path length covered by foraging mutant larvae is not different from controls. However, mutant larvae often stop, retract and turn, causing increased branching in their crawling paths.

Adult phototaxis and geotaxis behaviours are normal in mutants.

Hemizygous males and G9a^DD1/G9a^DD2 females both display a drastically slower response decrement during habituation to a "light-off jump reflex" compared to controls.

G9a^DD1 males show normal courtship learning behaviour, but their short-term and long-term courtship memory is reduced to 50% and 17%, respectively, of that of controls.

(Kramer et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

G9a^DD1, for^s has abnormal starvation stress response | female phenotype

(Anreiter et al., 2017)

G9a^DD1, for^s has abnormal feeding behavior | female phenotype

(Anreiter et al., 2017)

G9a^DD1, for^R has abnormal starvation stress response | female phenotype

(Anreiter et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

G9a^DD1 homozygosity eliminates the differences in foraging behavior between for^R homozygotes, for^s homozygotes or for^R/for^s heterozygotes. No difference in foraging success is observed between for^R homozygotes carrying wild-type G9a or G9a^DD1 alleles.

Flies double homozygous for G9a^DD1 and either for^s or for^R live significantly longer when starved than flies carrying wild-type alleles of G9a. The increase in starvation resistance is significantly greater in for^s than for^R homozygotes.

(Anreiter et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

G9a^DD1 is rescued by G9a^UAS.cKa/Scer\GAL4^hs.PB

(Kramer et al., 2011)

Partially rescued by

G9a^DD1 is partially rescued by G9a^UAS.cKa/Scer\GAL4⁴⁷⁷

(Kramer et al., 2011)

Not rescued by

G9a^DD1 is not rescued by G9a^UAS.cKa/Scer\GAL4⁴⁷⁷

(Kramer et al., 2011)

Comments

Expression of G9a^Scer\UAS.cKa using Scer\GAL4⁴⁷⁷ in a G9a^DD1 background rescues dendrite branching towards wild type levels.

Expression of G9a^Scer\UAS.cKa using Scer\GAL4⁴⁷⁷ in a G9a^DD1 background fails to restore normal larval locomotor behaviour.

Induced expression of G9a^Scer\UAS.cKa using Scer\GAL4^hs.PB after eclosion completely restores the short-term courtship memory defects shown by G9a^DD1 mutants.

(Kramer et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EHMT^DD1

(Lo and Chen, 2019, Anreiter et al., 2017, Kramer et al., 2011)

G9a^DD1

(Koemans et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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