FlyBase Allele Report: Dmel\gsb[491.RNAi.UAS]

General Information

Symbol

Dmel\gsb^491.RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0260925

Feature type

allele

Associated gene

Dmel\gsb

Associated Insertion(s)

Carried in Construct

P{UAS-gsb-RNAi.491}

Key Links

Transgenic product class

RNAi_reagent

(Marie et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Marie et al., 2010)

Mutagen

in vitro construct

(Marie et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-gsb-RNAi.491}

Cytology

Description

UAS regulatory sequences drive expression of 491bp of gsb coding sequence in an inverted repeat, generating dsRNA.

(Marie et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

gsb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Marie et al., 2010)

Detailed Description

Statement

Reference

Overexpression of gsb^{491.dsRNA.Scer\UAS} in the developing wing under the control of Scer\GAL4^Bx-MS1096 results in a severe perturbation of wing morphology.

Overexpression of gsb^{491.dsRNA.Scer\UAS} in post-mitotic neurons under the control of Scer\GAL4^elav-C155 does not generate a statistically significant difference in mEPSP amplitude and quantal content.

Overexpression of gsb^{491.dsRNA.Scer\UAS} in post-mitotic neurons under the control of Scer\GAL4^elav-C155 does not alter bouton size or 'satellite' bouton formation in mutant neuromuscular junctions.

(Marie et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

gsb^491.RNAi.UAS/Scer\GAL4^C57 is a non-enhancer of abnormal neurophysiology phenotype of GluRIIA^SP16

(Marie et al., 2010)

Scer\GAL4^elav-C155/gsb^491.RNAi.UAS is a non-enhancer of abnormal neurophysiology phenotype of Scer\GAL4^elav-C155, VGlut1^UAS.cDa

(Marie et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/gsb^491.RNAi.UAS is a suppressor of abnormal neurophysiology phenotype of GluRIIA^SP16

(Marie et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/gsb^491.RNAi.UAS is a non-suppressor of abnormal neurophysiology phenotype of Scer\GAL4^elav-C155, VGlut1^UAS.cDa

(Marie et al., 2010)

gsb^491.RNAi.UAS/Scer\GAL4^C57 is a non-suppressor of abnormal neurophysiology phenotype of GluRIIA^SP16

(Marie et al., 2010)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

gsb^491.RNAi.UAS/Scer\GAL4^C57 is a non-enhancer of neuromuscular junction phenotype of GluRIIA^SP16

(Marie et al., 2010)

Scer\GAL4^elav-C155/gsb^491.RNAi.UAS is a non-enhancer of neuromuscular junction phenotype of Scer\GAL4^elav-C155, VGlut1^UAS.cDa

(Marie et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/gsb^491.RNAi.UAS is a suppressor of neuromuscular junction phenotype of GluRIIA^SP16

(Marie et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/gsb^491.RNAi.UAS is a non-suppressor of neuromuscular junction phenotype of Scer\GAL4^elav-C155, VGlut1^UAS.cDa

(Marie et al., 2010)

gsb^491.RNAi.UAS/Scer\GAL4^C57 is a non-suppressor of neuromuscular junction phenotype of GluRIIA^SP16

(Marie et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of gsb^{491.dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 in a GluRIIA^SP16 heterozygous background significantly suppresses the homeostatic increase in presynaptic release normally observed in GluRIIA^SP16 mutants.

Expression of gsb^{491.dsRNA.Scer\UAS} under the control of Scer\GAL4^C57 does not change the synaptic homeostasis found in GluRIIA^SP16 heterozygotes.

Expression of gsb^{491.dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 in a GluRIIA^SP16 heterozygous background significantly reduces the synaptic depression normally observed in GluRIIA^SP16 mutants.

Expression of VGlut^Scer\UAS.cDa and gsb^{491.dsRNA.Scer\UAS} in post-mitotic neurons under the control of Scer\GAL4^elav-C155 causes a significant increase in average mEPSP amplitude. There is also a homeostatic decrease in presynaptic quantal content in these mutants.

(Marie et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

gsb^491.RNAi.UAS is rescued by gsb^UAS.cMa/Scer\GAL4^Bx-MS1096

(Marie et al., 2010)

Comments

Co-expression of gsb^Scer\UAS.cMa with gsb^{491.dsRNA.Scer\UAS} is able to significantly suppress the wing morphological defect found upon expression of gsb^{491.dsRNA.Scer\UAS} alone.

(Marie et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gsb^491.RNAi.UAS

gsb^{491.dsRNA.Scer\UAS}

gsb^{491.dsRNA.UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

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