FlyBase Allele Report: Dmel\Mi-2[GL00318]

General Information

Symbol

Dmel\Mi-2^GL00318

Species

D. melanogaster

Name

FlyBase ID

FBal0262785

Feature type

allele

Associated gene

Dmel\Mi-2

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00318}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00318}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00318

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^fl.SCA3.UAS

(Ashraf et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

(Nikalayevich and Ohkura, 2015)

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

(Nikalayevich and Ohkura, 2015)

spindle | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

(Nikalayevich and Ohkura, 2015)

Detailed Description

Statement

Reference

Expression of Mi-2^GL00318 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in significant increase in chromosome undercondensation and misalignment of condensed chromosomes as well as mitotic spindle defects in mature oocytes compared to wild-type, but the karyosome formation during prophase I in stage 12 or 13 maturing oocytes is unaffected.

(Nikalayevich and Ohkura, 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Mi-2^GL00318, Scer\GAL4^{VP16.mat.αTub67C} has oocyte phenotype, enhanceable by Rcc1^UASp.mCherry, Scer\GAL4^{VP16.mat.αTub67C}

(Nikalayevich and Ohkura, 2015)

Mi-2^GL00318, Scer\GAL4^{VP16.mat.αTub67C} has chromosome | adult stage | female phenotype, enhanceable by Rcc1^UASp.mCherry, Scer\GAL4^{VP16.mat.αTub67C}

(Nikalayevich and Ohkura, 2015)

Enhancer of

Statement

Reference

Mi-2^GL00318, Scer\GAL4^{VP16.mat.αTub67C} is an enhancer of oocyte phenotype of Rcc1^UASp.mCherry, Scer\GAL4^{VP16.mat.αTub67C}

(Nikalayevich and Ohkura, 2015)

Mi-2^GL00318, Scer\GAL4^{VP16.mat.αTub67C} is an enhancer of chromosome | adult stage | female phenotype of Rcc1^UASp.mCherry, Scer\GAL4^{VP16.mat.αTub67C}

(Nikalayevich and Ohkura, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of undercondensed chromosomes observed in mature oocytes expressing either Rcc1^{Scer\UAS.P\T.T:Disc\RFP-mCherry} or Mi-2^GL00318 RNAi under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is strongly increased by simultaneous expression of both of the transgenes, the undercondensed chromosomes are not only longer but also wider compared to controls and have higher median volume.

(Nikalayevich and Ohkura, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

35398