FlyBase Allele Report: Dmel\Cpsf6[HMS00113]

General Information

Symbol

Dmel\Cpsf6^HMS00113

Species

D. melanogaster

Name

FlyBase ID

FBal0263118

Feature type

allele

Associated gene

Dmel\Cpsf6

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00113}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS00113}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00113

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Tang et al., 2018)

female sterile, with Scer\GAL4^VP16.otu

(Yan and Perrimon, 2015)

lethal, with Scer\GAL4^nub-AC-62

(Yan and Perrimon, 2015)

lethal | female limited, with Scer\GAL4^dome-PG14

(Yan and Perrimon, 2015)

Phenotype Manifest In

autophagosome | larval stage | somatic clone | conditional, with Scer\GAL4^Act.PU

(Tang et al., 2018)

embryonic/larval fat body | larval stage | somatic clone | conditional, with Scer\GAL4^Act.PU

(Tang et al., 2018)

lipid droplet | larval stage | somatic clone | conditional, with Scer\GAL4^Act.PU

(Tang et al., 2018)

spermatid, with Scer\GAL4^VP16.bam

(Gallicchio et al., 2024)

Detailed Description

Statement

Reference

Larval fat body clones expressing Cpsf6^HMS00113 under the control of Scer\GAL4^Act.PU show an increase in lipid droplets and an impaired induction of autophagosomes (identified as mCherry-Atg8 puncta) in response to starvation.

(Tang et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Cpsf6^HMS00113, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal size | adult stage phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Cpsf6^HMS00113, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Phenotype Manifest In

Suppressor of

Statement

Reference

Cpsf6^HMS00113, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Cpsf6^HMS00113, Scer\GAL4^GMR.PU is a suppressor of autophagosome | larval stage phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Cpsf6^HMS00113, Scer\GAL4^GMR.PU is a suppressor of eye disc phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Cpsf6^HMS00113, Scer\GAL4^Act.PU is a suppressor of embryonic/larval fat body | somatic clone | larval stage phenotype of Cdk8^{UAS.ORF.Tag:HA}, Scer\GAL4^Act.PU

(Tang et al., 2018)

Cpsf6^HMS00113, Scer\GAL4^Act.PU is a suppressor of autophagosome | somatic clone | larval stage phenotype of Cdk8^{UAS.ORF.Tag:HA}, Scer\GAL4^Act.PU

(Tang et al., 2018)

Cpsf6^HMS00113, Scer\GAL4^Act.PU is a suppressor of embryonic/larval fat body | somatic clone | larval stage phenotype of Doa^{UAS.PS.Tag:FLAG}, Scer\GAL4^Act.PU

(Tang et al., 2018)

Cpsf6^HMS00113, Scer\GAL4^Act.PU is a suppressor of autophagosome | somatic clone | larval stage phenotype of Doa^{UAS.PS.Tag:FLAG}, Scer\GAL4^Act.PU

(Tang et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34804

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00113}attP2

NIG-Fly

HMS00113

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00113}attP2

Notes on Origin

Discoverer

Comments

Supplemental Dataset_S01.

(Yan and Perrimon, 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG7185^HMS00113

Cpsf6^HMS00113

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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